J0204-1-1 細胞骨格構造の制御と動的力学刺激に対する応答評価(生体振動計測とその応用(1))

概要

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In this study, a micro sensor-actuator device is deveroped to enforce dynamic stiulation and to detect change of mechanical properties by the stimulation induced in a cultured adhesive cell. The micro device utilizes a clamped-free beam type vibrator of which the vibration is excited by a piezoelectric ceramic. The device has an abilty to measure mechanical property of a cultured cell as compliance. It was mounted on a micro manipulator system, while dimensions and morphogeis of the cells were measured by an inverted phase contrast microscope system. Experimental studies have been carried out by using cultured normal human osteoblast (NHOst), because of its sensitivity to external mechanical stimuli. Cytoskeletal condition was controlled by repolymerization time after depolymerization treatment with a use of Cytochalasin D. The method has shown enough capability to detect difference of mechanical properties dueto changes of sytoskeletal stress fiber architecture in NHOst.
2009-09-12