S0201-2-1 3次元振動ステージを用いた培養細胞への力学刺激付加システム(マイクロ・ナノバイオメカニクス:細胞生物学への接近(2)刺激と応答)

概要

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In this study, a three-dimensional vibration stage was developed to enforce dynamic stimulation onto cultured cells seeded on a 35 mm culture dish. Three piezoelectric ceramics were attached on a newly designed V-shaped vibrator, so that it perform three-dimensional vibration up to around 80 μm in needed axial direction. Because of its simple structure, a sterilization treatment, which should be done before setting the vibration stage in a CO_2 incubator to avoid contamination, can be carried out without difficulty. Focusing on osteoblastic character, which is sensitive to external mechanical stimuli, a normal human osteoblast (NHOst) is stimulated by the developed three-dimensional vibration stage. In order to estimate effect of dynamic stimulation upon cultured cell, we define parameters of projected area and slederness ratio of the cultured NHOst. It is found that the vibration stimulation affects cell morphology.
社団法人日本機械学会の論文
2009-09-12