526 細胞種間における単離細胞核の力学的特性の比較(T10-2 マイクロ・ナノバイオテクノロジー(2):細胞内部の現象を捉える,大会テーマセッション,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

概要

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Local stiffness of cell nuclei isolated from vascular smooth muscle cells (VSMCs) and fibroblasts (FBs) were determined using an atomic force microscope (AFM). Nuclei were chemically isolated and attached to a cover glass in Hanks' balanced salt solution of room temperature. Then, the surface of a nucleus was observed using AFM, and force curves were obtained from the central region (8×8 μm) of the nucleus at the interval of 0.5 μm. From force-indentation curves, the distribution of local stiffness was determined in the central portion (4×4 μm) of the measurement area. Initial stiffness of the nuclei obtained from VSMCs and FBs were 0.43 nN/μm and 0.36 nN/μm, respectively; there was a tendency that the nucleus of VSMCs was stiffer than that of FBs, but the difference was not statistically significant. These results suggest that the difference in cell types does not affect much to the local surface mechanical properties of cell nucleus.
一般社団法人日本機械学会の論文
2008-08-02