817 メッシュ状Scaffoldを用いた靭帯再建用培養組織の作成に関する研究(GS-2 生体材料,研究発表講演)

概要

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Biodegradable polymer meshes were used for the construction of ligament-like tissue in vitro and effect of mesh size on the developed tissue was evaluated. Fibroblasts obtained from the rabbit anterior cruciate ligaments were seeded within collagen gel which was combined with PLLA mesh scaffolds having different mesh size (150 and 400μm). The fibroblast-collagen gel-scaffold complexes were cultured for 28 and 35 days under cyclic loaded conditions and, then, tensile tests and histological observation of thus reconstructed ligament-like tissues were performed. Cross-sectional area of both complexes decreased with culture period. Maximum load of the complexes decreased by approximately 20% after 35days, and were almost the same as the maximum load of scaffolds incubated under the same condition. However, histological observation revealed that collagen fibers and cells were aligned parallel to the long axis of complex by cyclic load, and the scaffold with larger mesh size was more suitable than the scaffold with small one for ligament-like tissue reconstruction.
社団法人日本機械学会の論文
2005-03-18