917 圧縮負荷に対する単一細胞のカルシウムイオンシグナル応答(GS-2 バイオエンジニアリング(4))

概要

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Intracellular Ca^<2+> response was observed after the application of compressive load to a single bovine aortic endothelial cell (BAEC). The cells loaded with a fluorescent dye fluo-4 were floated in Hanks' balanced salt solution of 37℃, and a single spherical cell was attached to the fine tips of a pair of micropipettes. Then, the cell was compressed by 2μm or 3μm by moving one of the micropipettes with a micromanipulator. Fluorescence intensity in the cell was observed by means of confocal laser scanning microscope. In the separate experiment, compressive load applied to cells was measured with a cantilever-type load cell. Compressive load induced transient 2-fold increase in the fluorescence intensity, and then the intensity gradually decreased to original level. Intracellular Ca^<2+> concentration reached its peak at approximately 19 and 11 second after the compression of 2μm and 3μm, respectively. The compressive load was approximately 0.24μN when a single BAEC was compressed by 2μm.
一般社団法人日本機械学会の論文
2004-03-17