T0201-1-5 基板への接着・伸展に伴う線維芽細胞の表面積および体積変化([T0201-1]細胞の構造と流れにおけるマイクロ・ナノスケール解析(1))

概要

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We investigated changes in surface area and volume of fibroblasts during cell spreading on their substrate. After cell membrane and cytoplasm were fluorescently stained, cells were seeded on seven cover glasses and cultured for 0, 1, 2, 3, 4, 5, or 6 hours. Then, cells were fixed and observed using a confocal laser microscope. From the 3D reconstructed fluorescence images, morphological parameters such as surface area, volume, and adhesion area were obtained. Surface area and adhesion area increased with culture time up to 4 h, thereafter these parameters remained unchanged. Surface area increased as adhesion area increased and shape index decreased. In contrast, cell volume remained unchanged up to 6h. These results suggest that a resistance to change in volume strongly affect cell deformation, whereas a resistance to change in surface area does not restrict cell deformation during spreading on glass substrate.
一般社団法人日本機械学会の論文
2010-09-04