1001 F-アクチンと微小管の破壊が線維芽細胞の圧縮特性に及ぼす影響(S03-1 細胞・生体分子のバイオメカニクス(1),S03 細胞・生体分子のバイオメカニクス)

概要

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Compressive properties of fibroblasts obtained from rabbit patellar tendons were determined after disrupting F-actin or microtubules with cytochalasin D and colchicine, respectively. Each floating cell was held between a pair of microplates; one microplate was flexible and used as a cantilever for force measurement, while the other was rigid (compressive plate). Then, the cell was compressed by moving the compressive plate at the rate of 1μm/s. The force required to deform the cells increased non-linearly as their deformation increased. The slope of force-deformation curves in the range of small deformation was steeper in colchicine-treated cells than in intact cells, while the shape of curves for cytochalasin-treated cells was similar to that for intact cells. However, the sensitivity of force measurement may not be enough to detect the load in the range of initial deformation. Future improvement of the test system might be required to determine the role of cytoskeletons in the compressive properties of single whole cells.
2007-09-07