730 線維芽細胞の引張特性に及ぼすF-アクチンと微小管破壊の効果(S08-2 細胞の構造とメカノバイオロジー(2),S08 細胞の構造とメカノバイオロジー)

概要

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Tensile properties of fibroblasts obtained from rabbit patellar tendons were determined after F-actin and microtubules were disrupted separately or simultaneously. Intracellular F-actin and microtubules were disrupted with cytochalasin D and colchicine, respectively. Each cell was attached to the fine tips of a micropipette and a glass microplate, and stretched at the rate of 6μm/s. The stiffness of cells significantly decreased by the disruption of F-actin, while microtubule disruption did not affect the stiffness, where the stiffness was defined by the slope of each load-elongation curve between 0 and 20μm elongation. The stiffness of cells treated with cytochalasin D and colchicine simultaneously was almost the same level as that of cells treated with cytochalasin D alone. These results suggest that F-actin is major component which is responsible for the tensile properties of fibroblasts and microtubules and interaction of F-actin and microtubules don't contribute to the tensile properties of fibroblasts.
社団法人日本機械学会の論文
2006-09-15