眼組織から分離した表皮ブドウ球菌スライム産生株の起炎性とスライム産生能の動態

概要

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Staphylococcus epidermidis is a bacterial flora in human conjunctiva. Certain strains can produce slime. We studied how the productivity of slime-producing strains would be affected with the change of growth environment. The infectivity of slime-producing strains were analysed in rabbit eyes. Eleven strains of Staphylococcus epidermidis were isolated from the human conjunctiva and were stained by safranine using microplate method and then checked slime-productivity. Two slime producing strains were observed. These 2 strains were subcultured for 5 generations in sheep blood agar and common agar to study the slime-producing activity. On the other hand, we inoculated the rabbit cornea by 100 μl of 107 cfu/ml solutions from slime-producing strains culture and observed the inflammation 24 hours after inoculation. These results of experiment suggest that slime-producing strain can be isolated in about 15～20% from Staphylococcus epidermidis of human conjunctiva. The slime producing activity dropped in the subculture in common agar but not so much in well-fed sheep blood agar. These results do indicate that the slime-producing strains possess infectivity to the rabbit cornea.
東京女子医科大学の論文
1999-06-25