Bradykinin 抗体の解析並びにこれを応用したbradykinin 測定法の検討

概要

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Anti-bradykinin rabbit serum was obtained by injecting bradykinin with complete Freund's adjuvant and the antibody was analysed by column chromatography, electrophoresis and ultra centrifugation and the antibody titer was estimated by bradykinin sensitized red cell haemagglutination technique. Brandykinin levels in blood was determined by bradykinin sensitized red cell hemagglutination inhibition test and the results obtained were as follows: 1) Antibody fraction reacting to bradykinin was eluted by DEAE cellulose column chromatography with 0.1 M phosphate buffer (pH 7.0) and the sensitized red cell haemagglutination titer of the fraction was 2560. 2) This fraction seemed to belong to 1gA by electrophoresis and the fraction was stable to heat. 3) By the analysis with ultra centrifugation the fraction was found to be consisted with two proteins: one is 6S (80%) and the other is 13S(20%). 4) Bradykinin levels in blood was determined by bradykinin sensitized red cell haemagglutination inhibition test and it was demonstrated that bradykinin level of blood in urticaria patients were about 10 holds higher (0.5-1.0 mcg/ml) than normal human sera. Also the bradykinin titer was found to be increased in the same sera by treating with trypsin.
日本アレルギー学会の論文
1967-04-30