40.サツマイモにおけるクマリン化合物の生合成と桂皮酸類オルト位水酸化酵素遺伝子のクローニング(口頭発表)

概要

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Coumarins are often found as the plant secondary metabolites in plants. They are thought to play important roles in plant defense due to their antimicrobial and antioxidative activity. We have identified the feruloyl-CoA ortho-(6'-)hydroxylase (AtF6'H1) in Arabidopsis, which catalyzes the key step of scopoletin biosynthesis. AtF6'H1 is classified into 2-oxoglutarate-dependent dioxygenase (2OGD) and exhibits the high substrate specificity for feruloyl-CoA. Arabidopsis mainly accumulates scopoletin. These results indicate that the substrate specificity of AtF6'H1 determines the accumulation patterns of the coumarins in Arabidopsis. In sweet potato (Ipomoea batatas L.) roots, both umbelliferone and scopoletin are accumulated in response to various stresses such as wounding and microbial infection. In order to identify the ortho-hydroxylase involved in formation of umbelliferone and scopoletin in sweet potato, we performed cloning and functional analysis of the AtF6'H1 homologs of sweet potato (Kokei No.14). The total sequences of the homologs were obtained by RT-PCR and RACE methods using cDNA from sweet potato roots as a template. The primers for cloning were designed according to the EST sequences of sweet potato, which are homologous to AtF6'H1.
2008-10-06