クロマトプローブ注入口付GC-MSを用いた有機リン系農薬及びその代謝物の簡易迅速分析法

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A rapid and simple method for the analysis of 19 organophosphorus pestcides and their metabolites in the blood was established by GC-MS with Chromatoprobe injector. This method was follwed, 100 μL of plasma and 900 μL of acetone was mixed for a few minutes and 5 min later the 2 μL of upper layer were loaded into the disposable microvial. Recovery results were divided into following 3 groups(table). The recoveries of first group ranged from 77% in fenitrothion to 145% in thiometon with less than a 20% RSD. The recoveries of second group ranged from 48% in phosalone to 162% in isofenphos-oxon with 30-72% RSD. The recovery of third group was 93% in EPN-oxon with 109% RSD. The whole time of analysis was about 40 min which include the sample preparation time for 10 min and the analysis time for 30 min. Thus the present method is easy and rapid. We think that the present method is one of the useful methods which organophosphorus pestcides and oxonmetabolites can be detected when the poisoning accident has happened. Table. Recoveries of 19 organophosphorus and these metaborics in plasma [table]
2002-01-24