固相抽出-HPLCによる柑橘類およびバナナ中のイマザリルの定量法について

概要

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A method for simple and rapid determination of imazalil in citrus fruits and banana was examined using solid-phase extraction column (2OH) and high performance liquid chromatography (HPLC). The imazalil was extracted from citrus fruits and banana with n-hexane-ethyacetate (9:1). A 10mL of the extract was passed throush Bond Elut (2OH) cartridge at a flow rate 1mL/min which was previously washed with 5mL of n-hexane-ethyacetate (9:1). The cartridge was washed with 8mL of n-hexane-ethyacetate (7:3) and air-dried under vaccum for 10min. Imazalil in cartridge was eluted with 2mL of HPLC mobile phase. The determination limits of imazalil in citrus fruits and banana were 0.1 μg/g. Recoveries of imazalil from citrus fruits fortifed at the levels of 1.25 μg/g and 2.50 μg/g and banana fortifed at the levels of 0.5 μg/g and 1.0 μg/g were 82.0-91.3%. The mean contents of imazalil in the marketed fruits were 0.57 μg/g in orange (n=19), 0.66 μg/g in lemon (n=18) and 0.74 μg/g in grapefruit (n=28). No imazalil was detected in sweetie (n=5) and banana (n=8).
日本食品化学学会の論文
2001-03-31