皮膚ノ吸收並ニ透過ニ就テ : 第2編皮膚ノ抗原(又ハ抗體)吸收ニ就テ

概要

論文の詳細を見る
To study the absorptiveness of the antigen or and-body from the skin the author used rabbits in the condition described in Part I, and healthy male guinea-pigs of approximately 250 g. They were fixed in a dorsal position and the hair of the abdominal part was cut with the scissors in the same way as described in Part I, the area of the experiment of the rabbits being 40 cm^2 and that of the guinea-pigs being 10.5 cm^2. The experimental abdominal parts were fomented with a certain antigen or and-body of constant quantity. The fomentation was produced by bringing absorbent cotton of constant area into contact with the skin surface without compressing or embrocating it, and dropping the antigen or anti-body thereon with a pipette. 1) The abdominal parts of some rabbits and some guinea-pigs were rubbed very slightly with cotton saturated with a physiologic salt solution. 2) Some were repeatedly slightly rubbed with cotton soaked in soap solution and cleaned by pouring on the physiologic salt solution. 3) Some were slightly rubbed several times with a small piece of cotton saturated with 0.2 cc of petroleum-benzine, and cleaned with cotton soaked in the physiologic salt solution. The fomentation of antigens or anti-bodies was performed only after the specimens had been dried from the previous treatments (1-3). A. Typhoid fever vaccine as an antigen. The test rabbits were fixed during the fomentation of 5 cc of typhoid fever vaccine and even after the fomentation had dried they were not set free. Each of them was given a metal collar during the examination, lest they lick oft the test solutions that had been applied. Blood was taken from each rabbit every other day and worth of aggultinine against typhoidbacillus was determined from the active serum over a period of 14 days. The results were as follows: a) The worth of aggultinine of normal healthy without previous treatment was the same both before and after the fomentation. b) The worth of aggultinine of rabbits after the previous treatment (1) increased sometimes but slightly. c) The worth of aggultinine of rabbits after the previous treatment (2) increased some what in most cases. d)The worth of aggultinine of rabbitsafter the previous treatment (3) increased noticerbly in an early stage. B. Depositing of living staphylococcus aureus in physiologic salt solution (a platinum loop of pure cultivation of staphylococcus into 1.0cc of physiologic salt Solution) as an antigen. Thirty minutes, one, three, six, twelve and twenty-four hours after the fomentation, three drops of blood were taken aseptically successively from the auricular vein of each rabbits. Up to six hours after the fomentation the blood taken from rabbits was in a state of fixation, and thereafter in a state of liberation. The separate bloods were then put into melted agar with 1.5 per cent citrate of soda and was cultivated in separate agar plates at 37°C, and the number of colonies determined. Twenty-four hours after the fomentation two inguinal glands of each rabbit were inoculated aseptically and a sterile emulsion of the glands was made by adding 0.3 cc of physiologic salt solution to it. A platinum loop of this emulsion was cultivated in separate agar plates at 37°C. for twenty-four hours and the number of coloines determind. The results are shown in the table below. [table] C. Toxine of diphtheria as an antigen. Each fixed guinea-pig was fomented with 2.0 cc of diphteria toxine, its least lethal dose being 1/500 cc, and after the fomentation had been dried the pig was set free and weighed every day. The guinea-pigs were dissected as soon as they died and remaining alive also vivisected on the tenth day. The control animal was fomented with a physiologic salt, solution. The table below gives the result [table] D. Immuno-serum of Typhoid as an anti-body. The rabbits was fomented with 5.0 cc of immuno-serum of typhoid. Thirty minutes, one, three, six, twelve and twenty-four hours after the fomentation, the blood was taken aseptically from the auricular vein up to six hours it was in a state of fixation, and thereafter in a state of liberation. The worth of aggultinine against typhoid-bacillus of the active serum was then determined for forty-eight hours. The results are cited in the table below. (The figures indicate the average worth.) [table] It was decided as a result of the above described experiments that the normal healthy skin of rabbits and guinea-pigs absorb neither antigen nor antibody, if these were brought into contact with the skin merely by fomentation. But if the skin is previously treated with a physiologic salt solution, soap solution or petroleum-benzine, the absorption of antigen or and-body is relativly easy, as those previous treatments would cause an abnormality of both quantity and quality of the sebaceous matter and the horny layer. (refer Part I) And it was proved by Ishikawa, a lecturer connected with our clinic, that the skin of rabbits after a previous treatment with petroleum-benzine accelerated an emigration of histiocyten to the upper layer of the cutis from the lower lager. So, it was easy to understand that the phagocitosis, transportation of pathological products and production of anti-body would also be raised by this previous treatment. If this were true, the active and passive immunization through the skin must be influenced favourably by the previous treatment especially with petroeum-benzine. On the other hand, deep invasion of living bacteria through the skin must be influenced unfavourably by the same previous treatment, as the bacteria would be phagocitized by emigrated histiocytens.
京都府立医科大学の論文

著者

青木敏一
京都府立醫科大學

皮膚ノ吸收並ニ透過ニ就テ : 第2編皮膚ノ抗原(又ハ抗體)吸收ニ就テ

スポンサーリンク

概要

著者

関連論文

スポンサーリンク

皮膚ノ吸收並ニ透過ニ就テ : 第2編 皮膚ノ抗原(又ハ抗體)吸收ニ就テ

スポンサーリンク

概要

著者

関連論文

スポンサーリンク

皮膚ノ吸收並ニ透過ニ就テ : 第2編皮膚ノ抗原(又ハ抗體)吸收ニ就テ