皮膚ノ吸收並ニ透過ニ就テ : 第3編皮膚ノ色素透過性ニ就テ

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With the object of procuring the fundamental idea as to the permeability of the skin, the author attempted to examine the permeability of the supravital skin-membrane, by means of one particular apparatus personally devised on his own plan. The supravital skin-membrane: Pieces of skin were excised from the abdomen of rabbits under the conditions stated in part I. (The animals were fixed in a dorsal position and the hair of the abdominal part was cut with scissors as carefully as cautioned in part I.) The subcutaneous fat of the skin piece was then removed as neatly as possible, to leave the skin consisting of the two layers; epithelium and corium, to which the author gave the name Skin-membrane", regarding it as a membrane of complicated structure. The Test apparatus in use: The chief apparatus was composed of a twine of L-shaped tubes open at both ends, each made by welding two glasstubes with different inside diameters, 1.6 cm and 0.8 cm respectively, autogenously at right angles. The author pasted a skin-membrane between the larger ends of the two L-shaped tubes so as to enable him to observe the transfer of a test solution from the epithelial side to the corium side under the given conditions. As auxiliaries to the apparatus, a fixer was in service to keep the skin-membrane tight between the glass tubes temporarily before the use of the main holder, which was placed round the chief apparatus to secure the constant close contact between the larger ends of the two L-shaped tubes. The method of Main Test. Four kinds of the skin-membranes made from the following four kinds of skin pieces, as excised from erch rabbit. (1) A piece of skin without any preliminary treatment. (the skin-membrane made from this piece of skin was termed by the author the "Skin-membrane I") (2) A piece of skin prelimiarily rubbed very slightly with cotton saturated with the physiologic salt solution. (〃"Skin-membrane II") (3) A piece of skin preliminarily slightly rubbed repeatedly with cotton saturated with the soap solution and cleaned afterwards by pouring over the physiologic salt solution. (〃"Skin-membrane III") (4) A piece of skin preliminarily slightly rubbed several times with cotton saturated with 0.2 cc of the petroleum benzine, and cleaned afterwards with cotton saturated with the physiologic salt solution. (〃"Skin-membrane IV") The excision was made in all cases after the specimens had been dried from the preliminary treatment. The author had before him a row of the four apparatuses, each with one specimen of the four kinds of the skin-membranes excised from the same one rabbit. The colouring matters in use were: Trypaflavin, Rhodamin B., Fuchsin S. and Iudigocarmin sic., each dissolved in water individually in Mol/50. 5.0cc of one solution of each of these four colouring matters, were put into the four apparatuses on the epithelial side and 5.0cc of the sterilized physiologic salt solution on the corium side, with a pipette, that is to say, four different tests were made on the given apparatuses, all using one colour solution at a time. After inserting a loose cotton stopper in each smaller end, the apparatuses were then left as they were in an incubator at the temperature of 37°C for four (4) hours. They were being observed macroscopically at intervales of 30 minutes to ascertain whether or not the transfer of colouring matter had taken place. After four (4) hours, the density of colouring matter in the physiologic salt solution was measured by a comparator (Zweistuflges Universalkolorimeter, nach E. Leitz), to ascertain the degree of the transfer, if any. Preliminary Tests. Prior to performing the above main tests, the preliminary tests were, of course, carried out in the following order: (1) On thickness of skin-membranes. (2) On life duration of skin-membranes. (3) On quantity of the solutions in use and any irregularities caused therein after the tests. (1) On thickness of skin-membranes: Reference is now made to the fact that each of the main tests was made on the assumption that the difference in thickness between one skin-membrane in use from another was within a permissible limit. The apparatuses pasted with the skin-membranes I, II, III & IV, as taken from the ten (10) different rabbits, were left dried at a constant temperature of 60℃. for ten (10) days. These dried skin-membranes were punched out, one by one, into a disk of 1.5 cm in diameter according to the author's own method, and the weight of each membrane-disk was taken, with the results shown in the following table. The author would add that his stipulation was based on the assumption that, thickness of such membrane-disk, the area and weight keeping same, must be of smallest irregularity, if not same. [table] From the test results obtained, it could be legitimately concluded that the differences in thickness among the skin-membranes taken from the same one rabbit, were much smaller than in the case where thicknesses of the skin-membranes taken from various different rabbits were taken into consideration for comparison, and such minor differences in thickness, as shown in the table, could be regarded within the permissible limit, so far as the tests now made were concerned. (2) On the life duration of skin-membranes. It was also necessary to ascertain the life duration of the supravital skin-membranes of rabbits. For this purpose, the apparatuses pasted with those other skin-membranes that had not been given any preliminary treatments (the skin membrane I) after being filled with the mentioned respective solutions of colouring matters on the epithelial side and the physiologic salt solution on the corium side, were put into an incubator at 37℃, and the permeation of the colouring matters through the healthy skin-membranes was being carefully watched, and the number of hours required for the penetration was taken with the following records. [table] It was discovered that the results obtained were almost correctly checked with Przyleeki's test record on the life duration of the frog skin-membrane, which was eight (8) hours. (3) On the quantity of the solutions used, and any irregularities caused therein after the tests: The apparatuses with the skin-membranes that had not been given no preliminary treatment (the Skin-membrane I), after being as much filled as with 5.0 cc of Aqua dest. on the epithelial side and 5.0 cc of physiologic salt solution on the corium side, were put into an incubator at 37°C. The quantity of each solution was measured at the end of the fourth, the twelfth and the twentyfourth hour. [table] From these test results, it could be deduced that there was no harm in taking it for granted that the density of the permeated colouring matter in the physiologic salt solution as in the case of the main test could give a correct basis for comparision on which the quantity of the permeated colouring matter in the same solution could be ascertained. Results of Main Test. The main test of the permeability of the skin-membrane was carried out on the solution of one colouring matter three times, thus having necessitated twelve tests on the solutions of four (4) different colouring matters. The test results were shown in the following table, the numerals given therein indicating the average of the records taken during the tests. A. Hours requined for the colouring matters to penetrated skin-membranes. [table] It was concluded from the above results, that the supravital healthy skin-membrane of a rabbit did not permit the penetration of colouring matter when a water solution of colouring matter was brought in to contact with it on its epithelial side and the physiologic salt solution on its corium side. But if the supravital skin-membrane was preliminarily treated with physiologic salt solution, soap solution or petroleum-benzine, the penetration of colouring matter occured relativly or very easily. The velocity of penetration and the penetrated quantity of colouring matter were largest after the preliminary treatment with petroleum-benzine, less after a preliminary treatment with soap solution and the least after the preliminary treatment with physiologic salt solution.
京都府立医科大学の論文

著者

青木敏一
京都府立醫科大學

皮膚ノ吸收並ニ透過ニ就テ : 第3編皮膚ノ色素透過性ニ就テ

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皮膚ノ吸收並ニ透過ニ就テ : 第3編 皮膚ノ色素透過性ニ就テ

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皮膚ノ吸收並ニ透過ニ就テ : 第3編皮膚ノ色素透過性ニ就テ