皮膚ノ吸收並ニ透過ニ就テ : 第1編皮膚ノ色素吸收ニ就テ

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It is already recognized that normal healthy skin becomes absorptive when treated with chemicals which destroy the epithelium or dissolve the lipoids or those soluble in fat or lipoids. Even indifferent substances, like unspecific albumen, may be absorbed by healthy skin after it has been embrocated thoroughly. It is also believed by some authors, though denied by others, that even perfectly healthy skin absorbs various colouring matters and microbes. It is often said that water-soluble acid colouring matters may be absorbed by a healthy normal skin. On the contrary, both colouring matters soluble in water and fat, and basic ones soluble in fat are not believed to be absorbable by a healthy normal skin, except sharlachrot. The fat-solubility of colouring matters seems unfavourable to the absorptivenoss of the skin. It is, of course, very important to take the affinity of the colouring matters to the tissue of the body into consideration in studying absorption by the skin. With this in mind, the author wanted to see: 1) the possibility of the absorption by the normal healthy skin when water-soluble colouring matters are brought into contact witli the skin surface without any external supplement, as an embrocation; 2) the condition which allows the absorption of the water-soluble ones by the healthy normal skin; and 3) the influence of cholesterin solubility upon absorption by the skin. The colouring matters chosen for this investigation were Fuchsin S (G.) and Indigocarmin sie. (M.), as water-soluble acids, and Rhodamin B (M.) and Trypa-flavin (L.) as water and cholesterin-soluble bases, -their colour tone being easy to catch macroscopically when absorbed extra-intestinally and excreted into the urine. The results of the preliminary examination were as follows: 1) It was necessary to apply four to eight times more of the bases than the acids to recognize their tints in the urine. 2) The measurement of the expansibility with gelatine when cited mathematically was: Trypaflavin>Rhodamin B>Fuchsin S>Indigocarmin sie. 3) The state of the solubility and the colour tone of the above described colouring matters when put into the following solvents (approximately Mol/50) were as follows: a) In water, Trypaflavin and Fuchsin S were dissolved almost completely, Rhodamin B less, Indigocarmin sie. the least. b) In 40% alcohol, Trypaflavin was dissolved almost completely, Fuchsin S and Rhodamin B less, Indigocarmin sie. the least. c) In 96% alcohol, they were scarcely dissolved. d) In olive-oil, they did not dissolve.e) In Petroleum-benzine, they did not dissolve. f) In petroleum-benzine with a little cholesterin, Fuchsin S and Indigocarmin sie., did not dissolve; Rhodamin B and Trypaflavin were slightly dissolved. The colour tone of Fuchsin S and Rhodamin B ehanged variously in those different solvents, while that of Indigocarmin sic. and Trypaflavin did not change noticeably.Method of main examination. Healthy adult male white rabbits of approximately 2Kg. were fixed in a dorsal position and the pair of the abdominal part was cut with scissors as close as possible and very earefully so as not to make any wound detectable with a magnifying-glass. The abdominal part of each rabbit then got a fomentation of water solution (aqua destillata), 40% alcohol solution or olive-oil emulsion of those four colouring matters mixed respectively in Mol/50. The fomentation was made by bringing absorbent cotton of constant area into contaet with the skin surface without compressing or embrocating it, and then dropping thereon constant quantity of the solutions or emulsions stated above. 1) The abdominal parts of some rabbits were compressed repeatedly with cotton saturated with physiologie salt solution (so as to make the state of the skin "Benetzung", so-called after Lichtwitz.) 2) Some were slightly rubbed repeatedly with cotton soaked in soap solution and then cleaned up by pouring on the physiologie salt solution. 3) Some were slightly rubbed several times with a small piece of cotton soaked in 0.2cc of ptroleum-benzine, and it was immediately blotted with cotton saturated with physiologie salt solution. 4) Some were slightly rubbed several times with a small piece of cotton saturated with 0.2cc of petroleum-benzine. 5) In some cases the fomentation was done with petroleum-benzine, that is, cotton of constant area was dampened with 5.0cc of petroleum-benzine by dropping with a pipette. The fomentation of colouring matters was performed only after the specimens had been dried after the these preliminary treatments except in (5), in which case it was done directly after the preliminary treatment. The author maeroscopieally observed the tint of the urine at successive hours, and he occasionally made an incision in the treated parts of the abdomen to detect the tint of the subcutaneous tissue. The following results, were obtained. 1) Normal healthy skin without preliminary treatments did not absorb the water solution, 40% alcohol solution or the olive-oil emulsion of the acid colouring matters (Fuchsin S and Indigocarmin sic.) nor the basic colouring matters (Rhodamin B and Trypaflavin) except a small amount of Trypaflavin olive-oil emulsion. 2) Water solutions of the four colouring matters were in many cases absorbed by the skin after the preliminary tratment (1). 3) All the water solutions, the alcohol solution and the olive-oil emulsion of the four colouring matters were absorbed by the skin after the preliminary trearment (2) with a few exceptions. 4) All the water solutions, the alcohol solution and the olive-oil emulsion of the four colouring matters were absorbed by the skin after the preliminary treatment (3). 5) All the olive-oil emulions of the four colouring matters were absorbed by the skin after the preliminary treatment (4). 6) The olive-oil emulsions of Rhodamin B and Trypaflavin were absorbed by the skin after the preliminary treatment (5), while those of Fuchsin S and Indigocarmin sic. were not absorbed at all. 7) The water solutions, the alcohol solution and the olive-oil emulsions of the four colouring matters were absorbed by the skin without the preliminary treatment when they were powerfully embrocated. 8) The positivity of the absorption seemed to depend upon the rate of fat removal and frequency of the rubbing. The quantity absorbed, and the velocity of the absorption observed from the density of the tint in urine and the time of its first appearance seemed to depend upon the character of the colouring matters, (i.e. their water solubility, their fat solubility and their affinity for body tissue, etc.) The more soluble the colouring matters and the more dissolved the solvents, the greater the skin absorption. And generally the more expansive the colouring matters, the quicker the absorption. The absorption of the water-and fat-soluble colouring matters was best when olive-oil was used as their solvent. The colour tone of the olive-oil emulsion of Fuchsin S and Rhodamin B changed while it was being absorbed into the skin, the cause of this changee being due to the different preliminary treatment. That is: 1) The change of the colour tone of Fuchsin S nerver occurred in a normal healthy skin, while that of Rhodamin B sometimes diffused after a long period. 2) A slightly diffused change of the colour tone of Fuchsin S and Rhodamin B, sometimes characterized by very heavy-toned points, was often observed an the skin surface after the preliminary treatment (1). 3) The colour tone of both Fuchsin S and Rhodamin B showed a slightly diffused change an the skin surface after the preliminary treatment (2) very heavy-toned points often being observed an it. 4) The colour tone of Fuchsin S and Rhodamin B showed a slightly diffused, change an the skin surface after the preliminary treatment (3). Heavier toned points were almost always visible. 5) The same change as (4) happened an the skin surface after the preliminary treatment (4). 6) The colour tone of Fuchsin S never changed on the skin surface after the preliminary treatment (5), while that of Rhodamin B showed a slightly diffused change. Heavier toned points were always observed on it. The microscopical findings in fixed or frozen specimens following invasion of the skin by the colouring matters were as follows: 1) Fuchsin S was not imbibed by the corium of normal healthy skin. 2) Because of the successive dyeings with water colouring solutions (the water of which froze) it was impossible to determine whether the colouring matter was really absorbed by the skin because of the preliminary treatment (2) or (3), or imbibed secondarily. It was, however, evident that the colouration of the hair-follicles and sebaceous glands wag caused by a 24 hour fomentation of the olive-oil emulsions of Rhodamin B or Trypaflavin. 3) It was proved in the rase of the fixed specimens that those heavy-toned points in the skin after the preliminary treatment (3) corresponded to small defects of the epithelium. 4) The fixed specimens made from the skin according to preliminary treatment (3) prior to the fomentation of the colouring matterd, included healthy portions. These showed a decrease of the horny layer-cells and defeets because of the previous treatment, even when compared with the healthy parts. These results can be summarized as follows: Normal healthy skin does not absorb water or alcohol solutions or olive-oil emulsion of soluble in water colouring matters even after long periods (8 hours), unless the process of contact with the skin surface is assisted by external supplements such as compression, rubbing etc.
京都府立医科大学の論文

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青木敏一
京都府立醫科大學

皮膚ノ吸收並ニ透過ニ就テ : 第1編皮膚ノ色素吸收ニ就テ

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皮膚ノ吸收並ニ透過ニ就テ : 第1編 皮膚ノ色素吸收ニ就テ

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皮膚ノ吸收並ニ透過ニ就テ : 第1編皮膚ノ色素吸收ニ就テ