皮膚ノ吸收並ニ透過ニ就テ : 第4編 皮膚ノ抗原(又ハ抗體)透過性ニ就テ
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概要
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The author wanted this time to study the permeability of the skin with an antigen or anti-body such as typhoid fever vaccine, depositing living staphylococcus aureus in a physiologic salt solution, diphtheria toxine or immuno-serum of typhoid fever in the same way as was done in the case of the Part III. 5.0 cc of one solution of each of these four solutions, were put into the four apparatusses, each with one specimen of the four kinds of the skin-membranes excised from the same one rabbit, on the epithelial side and 5.0 cc each of a sterilized physiologic salt solution on the corium side. Every act was perfomed as aseptically as possible and was tested up to four hours. The physiologic salt solution after a four hours test was called for the sake of convenice the "Transfer solution". A. The transfer of an antigenous substance included in the typhoid fever vaccine into the "Transfer solution", Twelve rabbits in the condition stated in part II, whose worth of aggultinine had previously been determined, were divided into four groups. Each rabbit belonging to the first was given an intravenous injection of 1.0 cc of the "Transfer solution" with the "Skin-membrane I". Rabbits belonging to the second group were given an intravenous injection of 1.0 cc of the "Transfer solution" with the "Skin-membrane II". The third group received an intravenous injection of 1.0cc of the "Transfer solution" with the "Skin-membrane III". In the case of the fourth group the "Transfer solution" with the "Skin-membrane IV" was injected. Blood was taken from each rabbit every other day and the worth of aggultinine against typhoid-bacillus was determined from the active serum over a period of 21 days. The same methods for determining fresh and stale vaccine derived from the same stock were repeated respectively four times and the results obtained are as follow: a) The worth of aggultinine of the first group sometimes slightly decreased but never increased. b) The worth of agglutinine of the second group remained unchanged in nearly half of the cases and increased from the 3rd to 13th day after the injection the other half of the cases. c) The worth of aggultinine of the third group increased from the 5th to 11th day after the injection in many cases, and that of a few cases remained unchanged. d) The worth of aggultinine of the fourth group noticeably increased in most cases from the 3rd or 5th day after the injection. It was found that in general the antigenous substance in a stale vaccine permeated the skin-membrane (which had been preliminarily treated) more than that included in fresh vaccine. B. The transfer of living staphylococcus aureus (one loop of pure cultivation of staphylococcus aureus in 1.0 cc of physiologic salt solution) into the "Transfer solution". Each 1.0 cc of the "Transfer solution" derived from the four apparatusses with skin-membranes of four kinds was cultivated in separate agar plates at 37°C up to 24 hours and the number of colonies determined. The same test was repeated three times. The results are shown in the table below. [table] N.B. In the above table: "Skin-membrane I" means skin-membrane without any preliminary treatment. "Skin-membrane II" means skin-membrane preliminarily treated with a physiologic salt solution. "Skin-membrane III" means skin-membrane preliminarily treated with a soap solution. "Skin-membrane IV" means skin-membrane preliminarily treated with petroleum-benzine. C. The transfer of diphtheria toxine (its killing dose being 1/500 cc) into the "Transfer solution". (i) Each healthy male guinea-pig of approximately 250 g was given an intra-abdominal injection of 4.0 cc of the "Transfer solution" derived from the four apparatusses with skin-membranes of four kinds. The guinea-pigs were weighed every day up to 10 days. They were disected as soon as they died and those remaining alive were vevesected on the tenth day. The results are cited in the table below. [table] N.B. I, II, III and IV in the table indicate the group of guinea-pig injected "Transfer solution" from the different skin-membrane as explained in the former table. (ii) Each remaining 1.0 cc of the "Transfer solution""the original solution was diluted with a physiologic salt solution and twofold, four-fold and eight-fold solutions were respectively gained. Each 0.1 cc of the original, twofold, four-fold and eight-fold solution, derived from the four appartusses with skin-membranes of four kinds, was injected intracutaneously into four separate abdominal parts of healthy male guinea-pigs of approximately 300 g. After the test was performed, it was found that (1) the skin of pigs injected with the solution derived from the apparatus with the "Skin-membrane I" (not preliminarily treated) showed no macroscopically traceable change; (2) The skin of some pigs injected with the solutions derived from the apparatus with the "Skin-membrane II" (preliminarily treated with a physiologic salt solution) showed no macroscopically traceable change, whill that of some of them showed hyperaemia and infiltration of various grades on the day following the injection; (3) The skin of pigs injected with the solutions derived from the appartus with the "Skin-membranes III & IV" (preliminarily treated with either a soap solution or petroleum-benzine) showed cutaneous reactions of various kinds (hyperaemia, infiltration or necrosis) on the day following the injection. D. The transfer of an anti-body included in the immuno-serum of typhoid fever into the "Transfer solution". The Retection of the Transfer of aggultinine against typhoid-bacillus of the "Transfer solution" derived from the four apparatusses with skin-membranes of four kinds was examined the same test was repeated three times each after two, four and six hour periods. The figures in the table below show average results. N.B. I, II, III and IV in the table indicate the different skin-membrane as explained in the former table. The author came to the conclusion as a result of this examination that an antigen or an anti-body such as typhoid fever vaccine, a deposit of living strphylococcus aureus in a physiologic salt solution, diphtheria toxine or immunoserum of typhoid fever do not permeate within four hours the normal healthy supravital skin-membrane of rabbit from epitherial side into corium side, if the antigen or andi-body is brought only into contact with the skin-membrane by means of the apparatus contrived by the author. But if the supravital skin-membrane is treated preliminarily with a physiologic salt solution, soap solution or petroleum-benzine, the penetration of the antigen or and-body is relatively easy to different expents.
- 京都府立医科大学の論文
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