P-58 植物培養細胞によるフラボノイド類の配糖化反応(ポスター発表の部)

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Flavonoids are phenolic compounds widely present in plants and foods of plant origin. Hesperetin and hesperidin are type of flavonoid and present in several vegetables and fruits. Hesperetin is an aglycon without rutinose sugar moiety of hesperidin. Hesperetin and hesperidin are the major flavones in the peels of oranges, Citrus reticulate and Satuma mandarin (Citrus unshiu Marc.). These compounds are known to have certain biological activities including an antioxidant property, anti-inflammatory and carcinogenic depression effects. These compounds are used as agents and food ingredients. Irrespective of such biological activities, the use of hesperetin and hesperidin has been limited, because of their low solubilities. Higher plants accumulate a wide range of glycosides as secondary metabolites, and are capable of conjugating sugar residues not only to endogenous metabolic intermediates but also to xenobiotics. To enhance the water-solubility of their flavonoids, we investigated the glycosylation using various kinds of plant cultured cells (Eucalyptus perriniana, Nicotiana tabacum cv. Bright Yellow, Phytolacca americana, Marchantia polymorpha etc.). Twenty kinds of the plant cultured cells were tested for the glycosylation toward hesperetin and hesperidin. As a result, eleven kinds of the plant cells had the ability of the glycosylation toward hesperetin and hesperidin. Their plant cells glycosylated at C7,C3', and C5-position of hydroxyl group in the substrate molecules (C7>C3'>C5). Eucalyptus perriniana cultured plant cells were hydrolysis hesperidin to hesperetin 7-O-β-D-glucoside.
天然有機化合物討論会の論文
2006-09-15

P-58 植物培養細胞によるフラボノイド類の配糖化反応(ポスター発表の部)

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