1 マナマコStichopus japonicusの新規神経突起伸展性ガングリオシド成分の構造と活性(口頭発表の部)

概要

論文の詳細を見る
On account of the variation for the sugar chain, glycosphingolipids (GSLs) are classified into cerebroside, sulfatide, ceramide oligohexoside, globoside, and ganglioside. Gangliosides, sialic acid-containing GSLs, are especially enriched in brains and nervous tissues, and are involved in the regulation of many cellular events. Recently, a number of GSLs have been obtained from the marine invertebrates such as echinoderms, poriferans, and mollusks. We have also been researching the biologically active GSLs from the echinoderms for elucidating the structure-function relationships of GSLs, and for developing the novel medicinal resources. In this time, we conducted the isolation and characterization of biologically active ganglioside from the sea cucumber Stichopus japonicus and could obtain two ganglioside molecular species, SJG-1 and SJG-2. The structures of these ganglioside were characterized on the basis of spectral (^1H-NMR, ^<13>C-NMR, ^1H-^1H COSY, HSQC, and negative ion FAB mass spectra) and chemical (methanolysis and methylation analysis) evidences. Namely, SJG-1 and SJG-2 are ganglioside molecular species possessing phytosphingosine-type long chain base and nonhydroxy fatty acid. The sugar moiety of SJG-1 is unique in respect of that a sialic acid directly binds to glucose of cerebroside, while that of SJG-2 is quite unique differing from those of gangliosides obtained from not only other sea cucumbers, but vertebrate animals. The gangliosides SJG-1 and SJG-2 shows neuritogenic activities toward the rat pheochromocytoma cell line PC12 cell at 10μM in vitro, especially SJG-2 reveals the most potent activities in the ganglioside which have been obtained from echinoderms in our laboratory.
2000-10-01

1 マナマコStichopus japonicusの新規神経突起伸展性ガングリオシド成分の構造と活性(口頭発表の部)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク