92(P06) 天然ヒノキチオールの抗菌性について(ポスター発表の部)

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We studied on the anti-bacterial effect of Hinokitiol (H) and its isomer γ-Thujaplicin (γ-T) which were purified from Aomori hiba oil against pathogenic Escherichia coli O-157, O-111 and Salmonella sp. The minimun inhibitory concentration s(MIC) of H against Vero toxin production strains of E. coli O-157 (n=3) and O-111 were 12.5μg/ml, and against other strains of E. coli (n=2) were 25μg/ml. MICs of H against Salmonella sp (n=8) were in the range of 6.3 to 25μg/ml. On the other hand, MICs of γ-T against E. coli and Salmonella were in the range of 6.3 to 25μg/ml and 12.5μg/ml, respectively. MICs of H against both species were the same concentration as other Gram negative rods. In the physiological saline containing 100μg/ml of H and approximately 10^6 of bacterial cells, 99% of E. coli type strain and 95% of Salmonella serogroup O-9 strain became extinct in half an hour. In the solution containing the same concentration of γ-T, extinction of both strains were 93% and 89%, respectively. No survived bacterial cell detected in these solution after 24 hours. In the nutrient broth containing 25μg/ml of H, the shape of both strains swelled or got longer, then certain condensation appeared at the both edges of rod cell or cells burst. In the case of 100μ/ml of H, bacterial cell did not swell but got longer and then became extinct as it was. The same was true of γ-T but these phenomena appeared later than H. These effects show that the anti-bacterial effect of H and γ-T were bactericidal but H is stronger than γ-T. The observation of the cells suggested that the bactericidal mechanism against Gram negative rods are the inhibition of cell division and denaturation of bacterial endoprotein.
天然有機化合物討論会の論文
1998-08-31

92(P06) 天然ヒノキチオールの抗菌性について(ポスター発表の部)

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