ビオチン欠乏妊娠マウスにおけるビオチン量およびビオチニダーゼ活性の変動

概要

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The effects of biotin deficiency on the concentration of "free biotin" (unbound and reversibly bound biotin) and "total biotin" (free and covalently bound biotin) and biotinidase activity in dams and their fetuses of mice were examined. Free biotin concentrations in the serum were significantly decreased at near term, after consumption of a biotin-deficient diet. The percents of free biotin to total biotin (free biotin rate) were 12% and 82% in the biotin-deficient and control groups, respectively. The liver free and total biotin concentrations in the biotin-deficient dams and their fetuses decreased significantly compared with the controls (50% in the dams and 60% in the fetuses). Free biotin rates were invariable in the biotin-deficient state, indicating continually the presence of the normal recycling of biotin. On the other hand, a large amount of biotin accumulated in the fetal skin and amniotic fluid in the control fetuses. No changes in the placenta biotin concentration were observed even in the biotin-deficient state. These findings suggest that biotin in these tissues plays a critical role in embryonic growth and differentiation. The liver biotinidase activity was low in the fetuses compared with their dams. However, there were no major differences in the serum or placenta between the biotin-deficient and control groups.
1994-07-25