Periodate Oxidation for Sulfated Glycosaminoglycans, with Special Reference to the Position of Extra Sulfate Groups in Chondroitin Polysulfates, Chondroitin Sulfate D and Chondroitin Sulfate K

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The optimum conditions for periodate oxidation of sulfated disaccharides were investigated to determine the position of extra sulfate groups on the saturated disulfated disaccharides obtained from chondroitin polysulfates, chondroitin sulfates D and K. Under the conditions: 2mM saturated disulfated disaccharide with 20mM sodium periodate at 37°in the dark, the uronic acid residue in the disulfated disaccharide from chondroitin sulfate D was rapidly and completely destroyed, whereas that in the disulfated disaccharide from chondroitin sulfate K was quite stable, even after removal of the sulfate group at the 4 position of the N-acetylgalatosamine residue. Accordingly, it was concluded that the structure of the disaccharide disulfate from chondroitin sulfate D is 2-acetamido-2-deoxy-3-O-(2-O-sulfo-β-D-glucopyranosyluronic acid)-6-O-sulfo-D-galactose and that from chondroitin sulfate K ; 2-acetamido-2-deoxy-3-O-(3-O-sulfo-β-D-glucopyranosyluronic acid)-4-O-sulfo-D-galactose.
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