Studies on the Mucopolysaccharides from Hagfish Skin

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概要

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• The mucopolysaccharides from the skins of hagfishes were isolated after pronase digestion and fractionation on a Dowex 1 column. The main mucopolysaccharides were found to be hyaluronic acid and dermatan polysulfate, from the results of electrophoresis, chemical analyses, digestions with hyaluronidase, chondroitinase-AC, -ABC and infrared spectroscopy. This dermatan polysulfate was homogeneous on electrophoresis and gel filtration. The molar ratio of galactosamine : uronic acid : sulfate was about. 1:1:2. On cellulose acetate electrophoresis in 0.2M calcium acetate, this dermatan polysulfate gave a single spot between SChS-B and ChS-H. Its infrared spectrum showed absorption bands for both axial and equatorial sulfate groups, and an absorption band at 800cm^<-1> differing from those of SChS-B and ChS-H. This mucopolysaccharide was not attacked by chondroitinase-AC, whereas it was degraded by chondroitinase-ABC and yielded a new spot "X", which moved more slowly than △Di-diS_D, in addition to spots corresponding to △Di-diS_B, △Di-4S, △Di-diS_E and △Di-6S. The material "X" was characterized as a trisulfated disaccharide : 2-acetamido-2-deoxy-3-O-(2 or 3-O-sulfo-β-D-gluco-4-enepyranosyluronic acid)-4, 6-di-O-sulfo-D-galactose, on the basis of chemical analysis and chondrosulfatase digestion. This mucopolysaccharide is a new type of dermatan polysulfate containing the trisulfated disaccharide unit : 2-acetamido-2-deoxy-3-O-(2 or 3-O-sufo-α-L-idopyranosyluronic acid)-4, 6-di-O-sulfo-D-galactose, two types of disulfated disaccharide units and two types of monosulfated disaccharide units.

• お茶の水女子大学の論文

著者

• Seno Nobuko

  Department Of Chemistry Faculty Of Science Ochanomizu University

• Akiyama Fumiko

  Department of Chemistry, Faculty of Science, Ochanomizu University

• Akiyama Fumiko

  Department Of Chemistry Faculty Of Science Ochanomizu University

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• Studies on the Mucopolysaccharides from Hagfish Skin
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