Development and characterization of an antibody specifically recognizing a mutant EGFR (L858R) protein expressed frequently in non-small cell lung cancer
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概要
- 論文の詳細を見る
L858R point mutation in exon 21 of the EGFR gene, accounting for approximately 40% of non-small cell lung cancer (NSCLC)-associated EGFR mutations, has been known to hyper-respond to gefitinib, a selective EGFR tyrosine kinase inhibitor. From this view point, it is important to detect EGFR mutations. Immunohistochemistry (IHC) is commonly used to analyze the molecular status of several clinical specimens. We have developed specific antibodies recognizing the mutant EGFR (L858R) protein and characterized the antibodies by ELISA, Western blot, immunocytochemistry, and immunohistochemistry. Using any of these evaluation methods, we found an antibody, AbyD02889, which could detect the EGFR (L858R) protein with specificity. AbyD02889 may be a useful tool to detect the EGFR (L858R) mutation of NSCLC in the clinical situation. IHC using the mutant-specific anti-EGFR antibody will be a powerful assay to predict or select subpopulation sensitive to EGFR-TKI.
- 近畿大学の論文
著者
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Kaniwa N
Department Of Genome Biology Kinki University School Of Medicine
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Nishio K
Department Of Genome Biology Kinki University School Of Medicine
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Nishio Kazuto
国立がんセンター研究所 薬効試験部耐性研究室
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Nishio Kazuto
Shien-laboratory National Cancer Center Hospital
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YOKOTE Hideyuki
Shien-Laboratory, National Cancer Center Hospital
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Yokote Hideyuki
近畿大学 医学部ゲノム生物学
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Yokote H
Shien-laboratory National Cancer Center Hospital
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Yokote Hideyuki
Department Of Genome Biology Kinki University School Of Medicine
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Yokote Hideyuki
Pharmacology Division National Cancer Center Research Institute
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Kawaishi Makoto
Shien-Lab, National Cancer Center Hospital, National Cancer Center Research Institute
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Kimura Hideharu
Respiratory Medicine, Kanazawa University Hospital
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Kasahara Kazuo
Respiratory Medicine, Kanazawa University Hospital
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Kawaishi Makoto
Shien-lab National Cancer Center Hospital National Cancer Center Research Institute
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Kawaishi Makoto
国立がんセンター研究所 薬効試験部耐性研究室
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Kasahara K
Respiratory Medicine Cellular Transplantation Biology Kanazawa University Graduate School Of Medicin
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Kasahara K
Kanazawa Univ. Kanazawa Jpn
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Kasahara Kazuo
国立がんセンター中央病院
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Kimura Hideharu
金沢大学 医学系研究科細胞移植学
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Kimura Hideharu
Respiratory Medicine Cellular Transplantation Biology Kanazawa University Graduate School Of Medicin
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Kimura Hideharu
国立がんセンター中央病院 支援施設
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Kasahara Kazuo
Respiratory Medicine Cellular Transplantation Biology Kanazawa University Graduate School Of Medicin
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Kasahara K
金沢大学 医学系研究科細胞移植学
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Kurashima Kazuyoshi
Respiratory Medicine Kanazawa Wakamatsu National Hospital
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