HUVECの形態およびVEカドヘリン発現に与える静水圧の影響

概要

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To study endothelial cell remodeling in response to hydrostatic pressure, human umbilical vein endothelial cells (HUVECs) were exposed to hydrostatic pressure of 25 and 100 mmHg and changes in morphology and expression of VE-cadherin were studied. After exposure to hydrostatic pressure, HUVECs exhibited elongated and tortuous shape without predominant orientation, together with development of centrally located, thick stress fibers. Particularly, HUVEs exposed to 25 mmHg exhibited more tortuous shape compared to those both under control conditions and 100 mmHg. HUVECs did not exhibit a multilayered structure unlike BAECs after exposure to hydrostatic pressure. Fluorescence staining demonstrated that VE-cadherin expression was lower under pressure than under control conditions. These results suggest that hydrostatic pressure may induce a decrease in VE-cadherin expression in HUVECs, leading to the formation of numerous randomly distributed intercellular gaps followed by the formation of tortuous shape.
社団法人日本生体医工学会の論文
2006-09-10