1011 局所力学刺激負荷コンドロサイトの弾性率変化とアクチンダイナミクス(S03-2 細胞・生体分子のバイオメカニクス(2),S03 細胞・生体分子のバイオメカニクス)

概要

論文の詳細を見る
Articular chondrocytes in vivo are continuously exposed to severe mechanical conditions and are supposed to exhibit reorganization of cytoskeletons, possibly altering mechanical properties of cells. In this study, the effect of local deformation on mechanical properties of chondrocytes was investigated using a micropipette aspiration technique, particularly focusing on actin remodeling. Repeat aspiration experiments showed that change in the Young's modulus of chondrocytes significantly depends on the aspiration rate. Ca^<2+> blocking experiments revealed that local deformation induces Ca^<2+>-dependent actin remodeling. Moreover, DIC technique showed that mechanical forces are rapidly transferred through cells and the induced strain converges within 2min. It is suggested that chondrocytes exposed to local stimulation may change mechanical properties associated with Ca^<2+>-mediated actin remodeling and intracellular stress transfer may be involved in chondrocyte mechanotransduction events.
社団法人日本機械学会の論文
2007-09-07