金基板上でのグアニン塩基の酸化電流に基づくDNA量の測定(<特集>生命科学と分析化学)

概要

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Electrochemical oxidation on a nucleic acid base, such as guanine, adenine, thymine, cytosine, was examined; only guanine could be oxidized on a gold electrode. Because deoxyguanosine, deoxyguanosine monophosphate were also oxidized on the electrode, guanine in DNA would be oxidized on the electrode. To prove DNA oxidation, thiolated-single strand DNA (ssDNA) was immobilized on a gold electrode, and electrochemical measurements were performed: differential pulse voltammetry (DPV) and chronoamperometry were examined. The voltammogram of DPV on an ssDNA-modified electrode had an oxidation peak at +1.17V vs. Ag/AgCl. The oxidation current was caused by the oxidation of guanine in the DNA-base. The electrical charge difference between a modified electrode and a bare electrode on chronoamperometry agreed with the guanine amount of ssDNA on gold, which was obtained from the result of a quartz crystal microbalance.
社団法人日本分析化学会の論文
2006-12-05