CHARACTERIZATION OF A NOVEL METALLOPROTEINASE IN DUVERNOY'S GLAND OF RHABDOPHIS TIGRINUS TIGRINUS
スポンサーリンク
概要
- 論文の詳細を見る
During the characterization of hemorrhagic factor in venom of Rhabdophis tigrinus tigrinus, so-called Yamakagashi in Japan, one of the Colubridae family, a novel metalloproteinase with molecular weight of 38kDa in the Duvernoy's gland of Yamakagashi was identified by gelatin zymography and by monitoring its proteolytic activity using a fluorescence peptide substrate, MOCAc-PLGLA_2pr(Dnp)AR-NH_2, which was developed for measuring the well-known matrix metalloproteinase (MMP) activity. After purification by gel filtration HPLC and/or column switch HPLC system consisting of an affinity column, which was immobilized with a synthetic BS-10 peptide (MQKPRCGVPD) originating from propeptide domain of MMP-7 and a reversed-phase column, the N-terminal amino acid sequence of the 38kDa metalloproteinase was identified as FNTFPGDLK which shared a high homology to Xenopus MMP-9. The 38kDa metalloproteinase required Zn^<2+> and Ca^<2+> ions for its proteolytic activity. In addition, the proteolytic activity was almost completely inhibited by BS-10, a MMP inhibitor, but not by the serine proteinase inhibitors, cysteine proteinase inhibitors and aspartic proteinase inhibitors.Together these results demonstrated that the 38kDa proteinase is a novel snake verom metalloproteinase (SVMP) containing HExGHxxGxxH motif which possesses high affinity to the BS-10 peptide, into its molecule, and the enzymatic properties are closed to that of MMPs. Based on the results obtained in the present study, we concluded that the 38kDa metalloproteinase is a novel metalloproteinase whose activity may be regulated by the cysteine switch mechanism, and could be classified as one of the matrix metalloproteinases rather than snake venom metalloproteinases.
- 日本トキシコロジー学会の論文
- 2006-05-22
著者
-
Minamino Naoto
Department Of Pharmacology National Cardiovascular Center Research Institute
-
MATSUDA Akira
Department of Hematology, Saitama International Medical Center, Saitama Medical University
-
Komori Koji
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Setsunan University
-
Hori Takamitsu
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hiroshima International University
-
AKIZAWA Toshifumi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Setsunan University
-
KONISHI Motomi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Setsunan University
-
MARUTA Yuji
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Setsunan University
-
TORIBA Michihisa
The Japan Snake Institute
-
SAKAI Atsushi
The Japan Snake Institute
-
NAKATANI Mitsuko
Department of Pharmacology, National Cardiovascular Center Research Institute
-
Maruta Yuji
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Setsunan University
-
Konishi Motomi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Setsunan University
-
Akizawa Toshifumi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Setsunan University
-
Matsuda Akira
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hiroshima International University
-
Matsuda Akira
Department Of Applied Chemistry Ehime University
-
Minamino Naoto
Department Of Molecular Pharmacology National Cerebral And Cardiovascular Center Research Institute
-
Nakatani Mitsuko
Department Of Pharmacology National Cardiovascular Center Research Institute
関連論文
- Lenalidomide is active in Japanese patients with symptomatic anemia in low- or intermediate-1 risk myelodysplastic syndromes with a deletion 5q abnormality
- Comparison of renal function under deliberate hypotension during epidural plus light-enflurane anesthesia and during enflurane anesthesia
- Comparison of Substrate Specificity of Matrix Metalloproteinases by Using Fragment Peptides Originating from MMP-2
- Comparison of Cleavage Site on Oxidized Insulin B-Chain and Synthetic Peptide originating from MMP-2 by the Metalloproteinase purified from Joro Spider, Nephila clavata
- Central Effects and Gene Structure of Calcitonin Receptor-Stimulating Peptides
- Reactions between Hydroxyl-Radical-Induced 7,8-Dihydro-8-oxo-2'-deoxyguanosine Precursor and the Spin Trap α-Phenyl-N-tert-Butylnitrone
- Damage Induced by Hydroxyl Radicals Generated in the Hydration Layer of γ-Irradiated Frozen Aqueous Solution of DNA
- Recent Trends in the Management of Graves' Hyperthyroidism in Japan : Opinion Survey Results, Especially on the Combination Therapy of Antithyroid Drug and Thyroid Hormone
- Construction of Peptidome Database and Its Application to Discovery of New Bioactive Peptides(Multidisciplinary Search for the Secrets of Eleven-Headed Kannon-Like Biological Functions of Peptide,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE Z
- Inhibitory Effect of Green Tea Polyphenols on Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP(Analytical Biochemistry)
- Genomic and Expression Analysis of Canine Calcitonin Receptor-stimulating Peptides and Calcitonin/Calcitonin Gene-related Peptide
- Identification of Novel Neuroendocrine Regulatory Peptide 1 and 2 by Peptidome Analysis Strategy
- PEPTIDOME Database and Identification of New Bioactive Peptides
- Peptidome Database and Identification of New Endogenous/Bioactive Peptides
- The Receptor-Gα Fusion Protein as a Tool for Ligand Screening : a Model Study Using a Nociceptin Receptor-Gα_ Fusion Protein
- Efficient Data Acquisition System for Peptidome Database
- Peptidome Project : Its Purpose And Methods
- CHARACTERIZATION OF A NOVEL METALLOPROTEINASE IN DUVERNOY'S GLAND OF RHABDOPHIS TIGRINUS TIGRINUS
- A Novel Bufadienolide, Marinosin, in the Skin of the Giant Toad, Bufo marinus
- MARINOIC ACID, A NOVEL BUFADIENOLIDE-RELATED SUBSTANCE IN THE SKIN OF THE GIANT TOAD, Bufo marinus
- Purification and Refolding of Recombinant Human proMMP-7 (pro-Matrilysin) Expressed in Escherichia coli and Its Characterization
- Purification of a Metalloproteinase in the Durvernoy's Glands of Rhabdophis tigrinus by Affinity Column based on Cysteine Switch Mechanism
- Excess Sludge Production and the Stable Operational Condition of a Submerged Membrane Activated Sludge Process
- Stable Operational Condition of Submerged Membrane Activated Sludge Process without Sludge Withdrawal
- THE EFFECT OF SLUDGE AGE ON DECOMPOSITION OF SLUDGE IN BATCH AEROBIC DIGESTION OF ACTIVATED SLUDGE ACCLIMATED TO GLUCOSE AND PEPTONE
- MEASUREMENT OF BOUND WATER IN EXCESS ACTIVATED SLUDGES AND EFFECT OF FREEZING AND THAWING PROCESS ON IT
- Graves' Hyperthyroidism Showing Transient Hypothyroidism during Interferon Therapy for Chronic Hepatitis Type C
- Treatment of Hyperthyroidism with a Small Single Daily Dose of Methimazole : A Prospective Long-Term Follow-Up Study
- Calcitonin Receptor-Stimulating Peptide and Calcitonin/Calcitonin Gene-Related Peptide : Their Evolutionary and Functional Relation in Mammals
- Blood Pressure-Depressing Activity of a Peptide Derived from Silkworm Fibroin in Spontaneously Hypertensive Rats
- Effect of Dietary Essential Fatty Acids on Pulmonary Metastasis of Ascites Tumor Cells in Rats(Biological)
- Freeze Concentration with Supersonic Radiation under Constant Freezing Rate - Effect of Kind and Concentration of Solutes -
- Parallelization of Finite Element Analysis Using Broadcast on Workstation Cluster
- Formation of Rough GaN Surface by Hydrogen Plasma Treatment and Its Application to Field Emitter : Semiconductors
- Monocyte Chemotactic Protein-1 Levels in the Vitreous of Patients with Proliferative Vitreoretinopathy
- Hepatocyte Growth Factor and Transforming Growth Factor-β Stimulate both Cell Growth and Migration of Human Gastric Adenocarcinoma Cells^1
- Synthesis of PRCGVPD-Containing Peptides as MMP-7 Inhibitors
- Chromosome Evolution and Speciation of Reptiles. I : Karyotype of the Japanese Mamushi, Agkistrodon blomhoffii blomhoffii (Viperidae, Crotalinae) with Special Regard to the Sex Chromosomes
- Amino Acid Sequence as Specific Peptide Substrate Targeted Membrane-Type 1 Matrix Metalloproteinase
- The Effect of the Freezing Column Diameter on Freezing and Thawing Treatment of Excess Activated Sludge
- COMPRESSION-PERMEABILITY CHARACTERISTICS AND EFFECT OF FREEZING AND THAWING PROCESS OF EXCESS ACTIVATED SLUDGE
- Transileocolic venous balloon dilatatio for the management of primary and recurrent portal venous stenosis after living donor liver transplantation in children
- Mesothelial cyst of the liver in a neonate
- Metal-Binding Ability of Human Prion Protein Fragment Peptides Analyzed by Column Switch HPLC
- Identification of New Ca^ Binding Site on Membrane Type Matrix Metalloproteinases by Using Synthetic Fragment Peptides
- Inhibitory Activities of Peptide-Conformers Originating from Matrix Metalloproteinase-7 against MMPs
- Reconstitution of the Na^+/H^+ Antiporter : A New Method for the Determination of H^+ Efflux from Na^+/H^+ Antiporter-Reconstituted Vesicles
- Identification and Functional Characterization of Kunitz-type Toxins from New Guinean Pseudechis australis
- A novel hypothalamic peptide, neuroendocrine regulatory peptide-2, regulates feeding behavior via the orexin system in the hypothalamus
- Proteolytic processing of neurosecretory protein VGF in the rat
- Peptidomics-based discovery of bioactive peptides
- The effects of prolactin-releasing peptide (PrRP) in energy metabolism and stress
- Global Identification of Peptide Processing Sites Implicated in the Regulated Secretory Pathway
- Multiple ligands for calcitonin receptor in mammals : calcitonin and calcitonin receptor-stimulating peptide
- TGF-β2, Tenascin, and Integrin β1 Expression in Superior Limbic Keratoconjunctivitis
- Simple Analysis of a Multi-Span Beam under Moving Loads with Variable Velocity
- Nilotinib as frontline therapy for patients with newly diagnosed Ph+ chronic myeloid leukemia in chronic phase : results from the Japanese subgroup of ENESTnd
- IS-4 The amplification mechanisms of FcεRIβ in the FcRγ-mediated human mast cells activation signal(Topics on IgE-FcεRI-Mast Cells)
- Peptidomics-Based Discovery of AMP-IBP5, an Antimicrobial Peptide Derived from Insulin-Like Growth Factor-Binding Protein 5
- Japanese-Western Consensus Meeting on Biomarkers : Executive Summary
- Compression characteristics of excess activated sludges treated by freezing-and-thawing process.
- Flooding and sonic velocity limit in a wetted-wall column at low pressure.