Epitope Mapping of a Monoclonal Anti-neurofilament Antibody in a Patient with Amyotrophic Lateral Sclerosis
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概要
- 論文の詳細を見る
An IgA monoclonal antibody, found to accumulate in spinal motor neurons of a patient with amyotrophic lateral sclerosis (ALS), reacted with the high molecular weight neurofilament protein (NF-H), and with an, as yet, unidentified 60 kD protein on the surface of neuroblastoma cells. The antibody binding had a pathophysiological effect as it inhibited proliferation of neuroblastoma cells in culture. To identify the reactive epitope, the antibody was tested for binding to overlapping recombinant fragments of NF-H generated by PCR and expressed in E. coli, and then to synthetic peptides spanning the reactive NF-H fragment using the Mimotope system. The epitope mapping studies revealed that the IgA recognizes the amino acid sequence EXAKSPXK in NF-H. This epitope contains a serine phosphorylation site that is critical for NF-H function. It is plausible that binding of the monoclonal antibody to the KSP epitope interferes with phosphorylation, disrupts normal cellular functions and ultimately leads to neuronldisease.
- 北里大学の論文
- 1995-06-30
著者
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Ogino Yutaka
Department Of Internal Medicine Kitasato University School Of Medicine.
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Latov Norman
Department of Neurology, College of Physicians and Surgeons, Columbia University.
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Sadiq Saud
Department of Neurology, College of Physicians and Surgeons, Columbia University.
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Sadiq Saud
Department Of Neurology College Of Physicians And Surgeons Columbia University.
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Latov Norman
Department Of Neurology College Of Physicians And Surgeons Columbia University.
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