Affinity of Human Anti-MAG Antibodies in Neuropathy

概要

論文の詳細を見る
Human IgM anti-myelin associated glycoprotein (MAG) antibodies from patients with neuropathy bind to oligosaccharide determinants shared by MAG and several other glycoconjugates in peripheral nerve. But the mechanisms responsible for the occurrence of anti-MAG antibodies are unknown. To determine whether the anti-MAG antibodies resemble "non-pathogenic" "natural autoantibodies" or whether they express high affinities similarly to autoantibodies in autoimmune disease, we measured the antibody affinities. The apparent affinities of human anti-MAG antibodies were determined by an ELISA system which measured free antibody concentration at equilibrium in solution. We found that the human antibodies bound to MAG with dissociation constants of between 2.5×10^<-10>M and 2.3×10^<-7>M. This result shows that IgM anti-MAG antibodies from patients with neuropathy, exhibit relatively high affinities which similar to high affinity antibodies derived by hyperimmunization. Their high affinities may be derived in the course of T-cell dependent immune response, and may contribute to their pathogenicity in patients with peripheral neuropathy.
北里大学の論文
1993-12-31