Effect of 8-Methoxypsoralen on the Crystalline Lens of Mammals : Changes in Epithelial Cells of the Crystalline Lens : A Preliminary Report
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概要
- 論文の詳細を見る
Regarding the influence of 8-methoxypsolaren (8-MOP), a photosensitive substance, on the crystalline lens of rats, the authors have reached the following conclusions, after examining the results of histological examinations in vivo and of ^3H-thymidine uptake in the cultured crystalline lens. 1. In vivo, 40mg of 8-MOP was injected into the rat abdominal cavity. After 28 hours of a black light (BLB) irradiation (3×10^<-1>J/mm^2), formation of small vacuoles was observed macroscopically in the equator area of the rat crystalline lens, while histological examinations revealed such changes as irregularity of the cell arrangement of the lens epithelia in the equator area, and deformation and pyknosis of the nucleus. 2. In the organ culture of the rat's crystalline lens, we succeeded in establishing a culture system that enables the photosensitized reaction of 8-MOP to cause the formation of an experimental nuclear cataract during the period between the 7th and 10th days of cultivation. 3. In the 9 day cultured rat crystalline lens with 8-MOP and BLB irradiation (3×10^<-1>J/mm^2) histological examination has shown that the epithelial cells have almost completely disappeared, a phenomenon that strongly suggests the presence of a primary change in the epithelial cells caused by a DNA metabolic disorder. 4. ^3H-thymidine uptake tests with 8-MOP and BLB irradiation (2×10^<-1>J/mm^2) revealed a decrease in the uptake close to 14% immediately before nuclear opacity in the 8-MOP experimental cataract group as compared with the control group, clarifying the presence of marked effects of the DNA metabolic disorder.
- 東海大学の論文
著者
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Obazawa Hajime
Department Of Ophthalmology School Of Medicine Tokai University
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MIKUNI Ikuo
Department of Ophthalmology, School of Medicine, Tokai University
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FUJIWARA Takaaki
Department of Ophthalmology, School of Medicine, Tokai University
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Mikuni Ikuo
Department Of Ophthalmology School Of Medicine Tokai University
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Fujiwara Takaaki
Department Of Ophthalmology School Of Medicine Tokai University
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