Measurement of Dietary Choline-Phospholipid Content by a Novel Phospholipase D-Triiodide Method
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概要
- 論文の詳細を見る
We developed a novel method that conveniently measures dietary cholinephospholipid content. Crude lipids extracted from dietary samples were reacted with phospholipase D from Streptomyces chromofuscus. The choline liberated from this reaction was then reacted with potassium triiodide, yielding choline periodide, which could be measured spectrophotometrically at 365 nm. This method proved to be more convenient than conventional assays, such as thin layer chromatography and high performance liquid chromatography. Our novel method is suitable for measuring many samples in single experiments.
- 日本ビタミン学会の論文
著者
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Ueda Aiko
Department Of Health Sciences Prefectural University Of Hiroshima
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Ueda Aiko
Department Of Health Science Hiroshima Prefectural Women's University
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Ishinaga Masataka
Department Of Food & Nutrition Hiroshima Women's University
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Ishinaga Masataka
Department Of Health Sciences Prefectural University Of Hiroshima
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HANADA Ai
Department of Health Sciences, Prefectural University of Hiroshima
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Hanada Ai
Department Of Health Sciences Prefectural University Of Hiroshima
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Ueda Aiko
Department of Health Sciences, Prefectural University of Hiroshima
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