トウモロコシの燐酸欠乏条件における特異的発現遺伝子に関する研究

概要

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Corn seedlings at 27 days were grown under phosphate starvation in experimental fields for long term of 25 years, and the gene expression was compared to the seedlings grown with phosphate application for the same term. Total RNA was extracted from these seedlings to isolate mRNA, and the purified mRNA was subjected to RT-PCR using arbitrary primers. The amplified cDNAs were electrophoresised on agarose gel, and genes specifically expressed in the phosphate-deficient seedlings were identified by the simplified differential display method. The phosphate starvation specific DNA bands were found and cloned. One of these clones was confirmed to be starvation specific by northern blotting. The sequence of this confirmed DNA fragment was analyzed by the cycle sequencing method. The result of the sequence homology analysis indicated that this clone has an unknown sequence.
明治大学の論文
2001-11-03