Measurement of Surface Topography of Endothelial Cell and Wall Shear Stress Distribution on the Cell

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概要

• 論文の詳細を見る

• Responses of endothelial cells to shear stress due to blood flow are basically heterogeneous. One of the reasons of the heterogeneity may be variation of the shear stress at individual cell level. In this study, we presented a method of three-dimensional cell shape measurement by confocal laser scanning microscopy, and determination of shear stress distribution on the surface of the cell by particle tracking velocimetry with a scale-up model. Thereby, shear stress distribution on the surface of statically cultured cells and flow-exposed cells was determined. From the results of two examples, it has been shown that the shear stress distribution on a cell had close correlation not only with the surface geometry of the cell but also with that of surrounding cells, and varied from cell to cell. The subcelluar distribution of the shear stress may provide important information to clarify the mechanisms of the mechanotransduction of endothelial cells.

• 一般社団法人日本機械学会の論文
• 2001-12-15

著者

• FUKUSHIMA Shuichiro

  Institute of Biomedical Engineering, Keio University

• Fukushima Shuichiro

  Institute Of Biomedical Engineering Keio University

• Oka Kotaro

  Department Of Biosciences And Bioinformatics Faculty Of Science And Technology Keio University

• Oka Kotaro

  Department Of System Design Engineering Keio University

• Tanishita Kazuo

  Department Of System Design Engineering Keio Univ.

• Kaibara Makoto

  Biopolymer Physics Laboratory Riken (the Institute Of Physical And Chemical Research)

• NAGATSU Ai

  Department of Mechanical Engineering, Keio University

• Tanishita Kazuo

  Department Of System Design Engineering

• Nagatsu Ai

  Department Of Mechanical Engineering Keio University

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