Improvement in the Viability of Cryopreserved Cells by Microencapsulation

スポンサーリンク

概要

• 論文の詳細を見る

• The advantages of microencapsulated cells over those of suspended cells were evaluated for improving viability in cryopreservation. Rat pheochromocytoma (PC12) cells were selected as the test biological cells and then microencapsulated in alginate-polylysine-alginate membranes. These microencapsulated PC12 cells were frozen by differential scanning calorimetry (DSC) at various cooling rates, from 0.5 to 10℃/min. Their latent heat was measured during freezing from 4 to -80℃. The post-thaw viability was evaluated by dopamine-concentration measurement and by trypan blue exclusion assay. Results showed that at cooling rates of 0.5 and 1℃/min, the latent heat of microencapsulated PC12 cells was lower than that of suspended cells. This lower latent heat is caused by the fact that the extra-microcapsule froze and the intracapsule remained unfrozen due to the formation of ice crystals in the extra-capsule space. The post-thaw viability of microencapsulated PC12 cells was improved when the cooling rate was 0.5 or 1℃/min, compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, maintaining the intra-microcapsules in an unfrozen state during freezing reduces the solution effect and thus improves the post-thaw viability.

• 一般社団法人日本機械学会の論文
• 2001-12-15

著者

• Tanishita Kazuo

  Department of System Design Engineering Keio University

• OKA Kotaro

  Department of Biosciences and Informatics, Keio University

• Oka Kotaro

  Department Of Biosciences And Bioinformatics Faculty Of Science And Technology Keio University

• Oka Kotaro

  Department Of System Design Engineering Keio University

• Matsumoto Yoshifumi

  Information Services International-dentsu Ltd.

• UJIHIRA MASANOBU

  Schools of Allied Health Sciences, Kitasato University

• Tanishita Kazuo

  Department Of System Design Engineering Keio Univ.

• MORINAGA Yukihiro

  Nipro Corporation

• Tanishita Kazuo

  Department Of System Design Engineering

• Ujihira Masanobu

  School Of Allied Health Sciences Kitasato University

関連論文

• P-03 Measurement of O_2 and NO by the Microcoaxial Electrode
• TED-AJ03-263 REGIONAL VARIETIES OF OXYGEN TRANSPORT IN THE CEREBRAL CORTEX BASED ON THE EXPERIMENTAL DATA
• A TEMPORAL-SPATIAL ANALYSIS OF TUMOR GROWTH AND ANGIOGENESIS(3D3 Biorheology & Microcirculation II)
• P-02 BLOOD FLOW DISTURBANCE BY ADMINISTRATION OF LIPOSOME IN BREAST CANCER
• Morphometric Changes of Small Airways using Microfocal X-ray Tomography(Cardiovascular Mechanics)
• Shear Stress Distribution on the Surface of Endothelial Cells during Flow-Induced Morphological Remodeling(Bioengineering)
• Functional repolarization of reconstructed hepatic organoid : Pumping function of bile canaliculi
• P-18 Effect of shear stress on three-dimensional microvessel network formation of endothelial cells
• Design of biodegradable Poly(DL-lactide-co-glycolide)(PLGA) microporous membrane for the reconstruction of three-dimentional hepatic tissues(3A3 Cellular & Tissue Engineering & Biomaterials III)
• Ductular morphogenesis and polarization of rat biliary epithelial cells in collagen gel sandwich culture(3A1 Cellular & Tissue Engineering & Biomaterials I)
• Albumin Permeability across Endothelial Monolayers under Long-Term Shear Stress(Bioengineering)
• Effect of Fluid Force on Vascular Cell Function
• Relationship between Microtubule Network Structure and Intracellular Transport in Cultured Endothelial Cells Affected by Shear Stress(Bioengineering)
• Effect of Shear Stress on the Formation of Three-Dimensional Angiogenesis Network(Micro- and Nano-biomechanics)
• Effect of Long-Term Shear Stress Exposure on Calcium Response and Morphology of Cultured Endothelial Cells(Bioengineering)
• Relationship between Energy-Dependent Macromolecule Uptake and Transport Granules in the Endothelial Cells Affected by Wall Shear Stress
• Intra-aneurysmal Flow Visualization by Phase Contrast MRI : Feasibility Study Using a Geometrically Realistic In Vitro Aneurysm Model.(Biorheology & Microcirculation)
• Native Response of C. elegans Encoded in Its Neuron Network : Cross-Disciplinary Physics
• Does a Randomly Organized Electrical Circuit Function as a Neuronal System?
• Development of heating method by microwave for sterilization of bone allografts
• 2-1 Effect of Fluid Frictional Force on ATP Synthesis of Vascular Cell
• Membrane Potential Measurement of Protoplasts Isolated from Vigna mungo Hypocotyl Using a Fluorescent Probe, diS-C_3-(5)
• 2-18 Vascular Cell Coordination Induced by Blood Flow(Proceedings of the 53rd Meeting of Japan Society of Physiological Anthropology)
• Physiological Basis of Earthworm Ventral Nerve Cord II. Characterization of Glutamate Receptor Subtypes
• Physiological Basis of Earthworm Ventral Nerve Cord I. Heterogeneity of Cellular Mechanisms Underlying Calcium Mobilization in the Median Giant Fiber
• Improvement in the Viability of Cryopreserved Cells by Microencapsulation
• Distribution of Wall Shear Stress in Right-Angle Branches during Laminar Flow(Bioengineering)
• Effect of Taper Angle on Wall Shear Stress at Tapered Right-Angle Branch in Laminar Steady Flow(Bioengineering)
• PHYSIOLOGICAL ROLE OF NO-cGMP SIGNALING IN EARTHWORM NERVOUS SYSTEM(Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• FUNCTION OF SEROTONIN IN SHORT- AND LONG-TERM MEMORY INDUCED BY CLASSICAL CONDITIONING OF THE EARTHWORM(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• NO/cGMP SIGNALING PATHWAY IN THE TERMINAL ABDOMINAL GANGLION OF THE CRICKET(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Serotonin-induced nitric oxide production in the ventral nerve cord of the earthworm, Eisenia fetida
• Visualization of nitric oxide production in the earthworm ventral nerve cord
• Surface Charge Density Estimation of Vigna mungo Protoplasts Using a Fluorescent Dye, 9-Aminoacridine
• Effects of External Ca^ on K^+ Permeability of the Elongating Region of Mung Bean Roots under High KCI Stress
• 2-1 Effect of Fluid Frictional Force on Vascular Cell Function(Proceedings of the 51st Meeting of Japan Society of Physiological Anthropology)
• Intravascular Inhomogeneous Distribution of Oxygen in Arterioles of Rat Cremaster Muscle
• Homeostasis of Circulatory System Maintained by Transferome(Special lecture,Proceedings of the 55th Meeting of Japan Society of Physiological Anthropology)
• Secondary Flow Augmentation during Intermittent Oscillatory Flow in Model Human Central Airways
• Measurement of Surface Topography of Endothelial Cell and Wall Shear Stress Distribution on the Cell
• Systems Biology for Neuroscience(Exploration of the Biological Intelligence Through Synergistic Contributions of Biology and Engineering,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
• Does a Randomly Organized Electrical Circuit Function as a Neuronal System?

もっと見る 閉じる

スポンサーリンク