加齢に伴う牛皮膚組織コラーゲンの抽出性の変化と得られた標品の生化学的特性

概要

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We examined four ways for collagen extraction from bovine skin tissue at different ages. From fetus and new born bovine skins, 50% collagen was extracted by 0.5 M acetic acid treatment. From the 3-month and 18-month old bovine, collagen was able to be extracted by pepsin or proctase digestion. Collagen was hardly extractable from 8-years old skin, unless alkaline extraction was applied. Such different profiles of extractability may reflect change of collagen crosslinks during aging. Acid labile, reducible crosslinks were found in fetus and new born bovine skin. Pyridinoline was detected in the bovine skin, elder than 3 month, and other crosslink, pentosidine, was exclusively detected in the 8 years-old bovine skin. We could not find any difference in amino acid compositions and denaturation temperature among the collagen specimens prepared from differently aged bovine tissues. However, the features of fibril formation were characteristically different from each other.
日本結合組織学会の論文
1999-03-25