Isolation and Characterization of Matrix Associated Region DNA Fragments in Rice (Oryza sativa L.)

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概要

• 論文の詳細を見る

• To investigate the interactions between chromosomal DNA and nuclear matrices in higher plants, matrix associated regions (MARS) of rice (Oryza sativa L.) DNAs were cloned. First, we prepared nuclear matrices from isolated nuclei by digesting them with EcoRI and then extracting with 2 M NaCl. About 6% of the total DNA remained in the nuclear matrices after this digestion and extraction. The residual DNA fragments in the nuclear matrices were cloned. Some of the cloned DNA fragments showed binding to certain nuclear proteins. One of the MAR fragments contained sequences related to known consensus motifs and a hairpin loop structure. A method is presented for isolation of matrix associated region (MAR) DNAS from plant cells.

• 日本植物生理学会の論文

著者

• NOMURA Koji

  Biochemical Research Laboratory, Ezaki Glico

• Saito W

  Tokyo Inst. Technol. Tokyo Jpn

• Saito Wataru

  Institute Of Agriculture And Forestry University Of Tsukuba

• Inoue M

  Biotechnology Institute Akita Prefectural University

• Masuda Kiyoshi

  Graduate School Of Agriculture Hokkaido University

• Masuda Kiyoshi

  Department Of Botany Faculty Of Agriculture Hokkaido University

• Ono Kimiyo

  Institute Of Biological Science University Of Tsukuba

• Masuda Kyojiro

  Department Of Bioiogy Faculty Of Science Toyama University

• Nomura Koji

  Institute of Agriculture and Forestry, University of Tsukuba

• Moriyama Hiromitsu

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

• Takahashi Seiko

  Division of Cell Biology, Akita Prefectural College of Agriculture

• Inoue Masayasu

  Division of Cell Biology, Akita Prefectural College of Agriculture

• Saito W

  Toshiba Corp. Kawasaki Jpn

• Nozawa Koji

  Department Of Electrical Engineering Faculty Of Engineering Kanagawa University

• Nomura K

  Institute Of Agriculture And Forestry University Of Tsukuba

• Moriyama Hiromitsu

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

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