cis-Regulatory Elements Responsible for Quantitative Regulation of the Rice Seed Storage Protein Glutelin GluA-3 Gene
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概要
- 論文の詳細を見る
The 897 bp 5' flanking region of the rice storage protein glutelin gene (GluA-3) was analyzed to define cis-regulatory elements responsible for the seed specific expression by progressive 5' deletions and gain-of-function experiments using a truncated CaMV ^<35>S promoter (-90/+9). These constructs were transcriptionally fused either to the firefly luciferase (LUX) or β-glucuronidase (GUS) reporter gene and introduced into tobacco. It was shown that a 121 bp sequence between -437 and -317 from the transcriptional start site is important for the quantitative regulation of the GluA-3 gene expression. This region contained at least two functionally different cis-regulatory elements. One acts as a general positive element and the other is involved in a specific negative regulation in non-seed tissues.
- 日本植物生理学会の論文
著者
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Takaiwa Fumio
Department of Biotechnology, National Institute of Agrobiological Resources
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Takaiwa F
National Inst. Of Agrobiological Sci. Ibaraki Jpn
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Takaiwa Fumio
National Institute Of Agrobioiogical Resources
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Yoshihara Toshihiro
Department of cell Biology, National Institute of Agrobiological Resources
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Takaiwa Fumio
Department Of Biotechnoiogy Central Research Institute Of Electric Power Industry
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Yoshihara Toshihiro
Department Of Cell Biology National Institute Of Agrobiological Resources
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