Regeneration of variant plants from rice (Oryza sativa L.) protoplasts derived from long term cultures.
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概要
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Whole plants were obtained efficiently from protoplasts of calluses which were induced from diploid seed (2n=24) and then sub-cultured on MS solid medium for 7-10 months. Agronomic characters and chromosome number were studied in the regenerated plants. Wide variation of agronomic characters such as plant height and heading date was found among the regenerated plants cultivated in the field. In 7 plants in which the chromosome number was determined, 4 tetraploids (2n=48), 2 aneuploids (2n=46) and 1 diploid were observed. The chromosome number was different between plants regenerated from a single protoplast clone, i.e. one was tetraploid (2n=48) and the other was aneuploid (2n=46).
- 日本遺伝学会の論文
著者
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Takaiwa Fumio
Department Of Biotechnoiogy Central Research Institute Of Electric Power Industry
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OONO Kiyoharu
Department of Cell Biology, National Institute of Agrobiological Resources
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KIKUCHI Shoshi
Department of Cell Biology, National Institute of Agrobiological Resources
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KANDAI Michie
Department of Cell Biology, National Institute of Agrobiological Resources
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- Restriction fragment length polymorphism of glutelin from cultivated rice (Oryza sativa L.).
- Regeneration of variant plants from rice (Oryza sativa L.) protoplasts derived from long term cultures.