RNA synthesis in embryo axes of germinating pea seeds
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概要
- 論文の詳細を見る
RNA synthesis during germination was investigated by labeling pea embryo axes or seedling roots with radioactive uridine or adenosine. The results indicated that all RNA species of pre-rRNAs (ribosomal precursor RNAs), rRNAs, heterodisperse-type RNA and 4-5S low molecular weight RNA were synthesized from the 6th to 64th hour of the period examined. At the very early stage of germination, some conspicuous labeling of the heterodisperse-type RNA was observed after pulse-labeling. There was no great difference in the labeling patterns of various RNA species with regard to other later stages. When embryo axes were labeled for 1 hr with ^3H-adenosine from the 16th hour, about 25% of the labeled whole cell RNA was retained on the membrane filter. The ratio of labeled poly(A)-containing RNA, however, decreased as germination proceeded. The poly(A)-containing RNA sedimented heterodispersely with a mean value of about 20S in a sucrose density gradient; this size-distribution did not vary throughout germination.
- 日本植物生理学会の論文
著者
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Takaiwa Fumio
Department of Biotechnology, National Institute of Agrobiological Resources
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Tanifuji Shigeyuki
Department of Botany, Faculty of Science, Hokkaido University
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Tanifuji Shigeyuki
Department Of Botany Faculty Of Science Hokhaido University
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Takaiwa Fumio
Department Of Botany Faculty Of Science Hokhaido University
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Takaiwa Fumio
Department Of Biotechnoiogy Central Research Institute Of Electric Power Industry
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