Differential replication of satellite and main band DNA during early stages of callus formation in carrot root tissue
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概要
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DNA synthesis during the early stages of callus formation was examined in carrot root slices cultured on an agar medium containing 2,4-D. During the first 12 hr of culture, only a low level of ^3H-thymidine was incorporated into DNA, after which the incorporation rapidly increased and reached a maximum at about 48 hr, then gradually decreased. CsCl density centrifugation of total tissue DNA indicated that a satellite DNA with a buoyant density of about 1.712 g/ml replicates in the early phase of the DNA synthetic period, then the main band DNA with a buoyant density of 1.695-1.700 g/ml starts its replication and continues to be produced throughout the synthetic period. The labeled satellite DNA, as well as the labeled main band DNA, was localized mainly in the subcellular fraction of 1,000 ×g sediments obtained from tissue homogenates. The patterns of cellular localization were not modified by the addition of Triton ×-100 to the initial homogenates. A considerable portion of the labeled satellite DNA was found in nuclelase-resistant chromatin subunits after limited digestion of the isolated chromatin with micrococcal nuclease.
- 日本植物生理学会の論文
著者
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Tanifuji Shigeyuki
Department of Botany, Faculty of Science, Hokkaido University
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Tanifuji Shigeyuki
Department Of Botany Faculty Of Science Hokhaido University
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Yakura Kimitaka
Department of Biology, Faculty of Education, Kanazawa University
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Hase Youko
Department of Botany, Faculty of Science, Hokkaido University
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Yakura Kimitaka
Department Of Biology Faculty Of Education Kanazawa University
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Yakura Kimitaka
Department Of Botany Faculty Of Science Hokkaido University
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Hase Youko
Department Of Botany Faculty Of Science Hokkaido University
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