Efficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous Plants
スポンサーリンク
概要
- 論文の詳細を見る
A series of chimeric promoters for higher-level expression of foreign genes in plants was constructed as fusions of a gene for β-glucuronidase (GUS) with the terminator of a gene for nopaline synthase (nos) or of the cauliflower mosaic virus (CaMV) ^<35>S transcript, and the strength of these promoters was assayed in transient and stable expression systems in tobacco and rice. As parts of these promoters, the CaMV ^<35>S core promoter, three different 5'-upstream sequences of the ^<35>S promoter, the first intron of a gene for phaseolin, and a 5'-untranslated sequence (Ω sequence) of tobacco mosaic virus were used in various combinations. In tobacco and rice protoplasts, all three fragments of the ^<35>S promoter (-419 to -90, -390 to -90 and - 290 to - 90, relative to the site of initiation of transcription), the intron, and the Ω sequence effectively enhanced GUS activity. Some chimeric promoters allowed levels of GUS activity that were 20- to 70-fold higher than those obtained with the ^<35>S prornoter in pBI221. In tobacco protoplasts, the two longer fragments of the ^<35>S promoter were more effective than the shortest fragment. In rice cells, by contrast, the shortest fragment was as effective as the two longer ones. The terminator of the ^<35>S transcript was more effective than that of the nos gene for gene expression. In transgenic tobacco plants, a representative powerful promoter, as compared to the ^<35>S promoter, allowed 10- and 50-fold higher levels of expression on average and at most, respectively, with no clear qualitative differences in tissue- and organ-specific patterns of expression. When the representative promoter was introduced into tobacco with a gene for luciferase, the autofluorescence of detached
- 日本植物生理学会の論文
著者
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HIROCHIKA Hirohiko
National Institute of Agrobiological Resources
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Ohashi Yuko
National Institute of Agrobiological Resources
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Nakamura Shigeo
Miyagi Prefectural Agriculture And Horticulture Research Center
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Mitsuhara Lchiro
National Institute Of Agrobiological Resources
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UGAKI Masashi
National Institute of Agrobiological Resources
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HONKURA Ryoso
Miyagi Prefectural Agriculture and Horticulture Research Center
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MURAKAMI Taka
National Institute of Agrobiological Sciences
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OHSHIMA Masahiro
National Institute of Agrobiological Resources
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Ohshima Masahiko
National Institute of Agrobiological Resources
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Gotoh Yoko
National Institute of Agrobiological Resources
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Katayose Yuichi
National Institute of Agrobiological Resources
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Nakamura Shigeo
National Institute of Agrobiological Resources
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Nishimiya Satoshi
National Institute of Agrobiological Resources
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Ueno Keiichiro
National Institute of Agrobiological Resources
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Mochizuki Atsushi
National Institute of Agrobiological Resources
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Tanimoto Hideo
National Institute of Agrobiological Resources
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Tsugawa Hidehito
National Agriculture Research Center
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Otsuki Yoshiaki
National Institute of Agrobiological Resources
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Mochizuki Atsushi
National Institute For Agro-environmental Sciences
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Gotoh Yasunari
Dept.mol.gen. Niar
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Ohashi Yuko
Plant-microbe Interaction Research National Institute Of Agrobiological Sciences
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Ohashi Yuko
National Inst. Agrobiol. Resources:crest Of Japan Science And Technology Corporation
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Ugaki Masashi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo:de
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Ugaki M
Laboratory Of Applied Entomology Faculty Of Agriculture The University Of Tokyo:(present Adress)depa
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Ugaki Masashi
Laboratory Of Bioresource Technology Graduate School Of Frontier Sciences The University Of Tokyo
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Murakami T
Microbiological Resources And Technology Laboratories Meiji Seika Kaisha Ltd.
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Murakami Taka
National Institute Of Agrobiological Resources
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Ueno Keiichiro
National Institute Of Agrobiological Resources:kagoshima Biotechnology Institute
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Tanimoto Hideo
National Institute Of Agrobiological Resources:osaka Prefectural Agricultural And Forestry Research
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Hirochika Hirohiko
National Inst. Of Agrobiological Sciences
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Hirochika H
National Inst. Agrobiological Sci. Ibaraki Jpn
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Tsugawa Hidehito
National Agriculture Research Center:aomori Green Biocenter
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Mochizuki Atsushi
National Institute Of Agrobiological Resources:tohoku National Agricultural Experimental Station
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Otsuki Yoshiaki
National Institute Of Agrobiological Resources:national Agriculture Research Center
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Hirochika Hirohiko
National Inst. Agrobiological Sci. Ibaraki Jpn
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Nishimiya Satoshi
National Institute Of Agrobiological Resources:ibaraki Horticultural Research Station
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Katayose Yuichi
National Inst. Of Agrobiological Sciences
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Mochizuki Atsushi
National Inst. Basic Biology
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Nakamura Shigeo
Miyagi Prefectural Agricultural Research Center
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Honkura Ryoso
Miyagi Prefectural Agricultural Research Center
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