Insect Resistance in Transgenic Chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis
スポンサーリンク
概要
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We introduced a modified delta-endotoxin gene, modified crylAb (mcbt) of Bacillus thuringiensis, which displays a specific biological activity against lepidopteran insects into chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura]. The chrysanthemum cultivar 'Shuho-no-chikara' was transformed using a disarmed strain of Agrobacterium tumefaciens, EHA 101, carrying a binary vector, pIGl2lmcbt that harboured a mcbt gene encoding an insecticidal crystal protein (ICP) fragment of B. thuringiensis var. kurstaki HD-1. Leaf discs were co-cultured with Agrobacterium and thereafter cultured on the callus induction medium containing G418. A total of 317 shoots were regenerated from 3,618 leaf discs on the regeneration medium (8.8%). The mcbt gene was detected in all the regenerated plantlets by Southern blot analysis. The accumulation of CrylAb ICP in 20 transformed lines, selected at random, was confirmed by Western blot analysis. The level of accumulation of CrylAb ICP ranged from 10.5 ng to 80 ng per 50 μg total soluble protein (from 0.021 to 0.16% of the total protein). Insect bioassay was conducted using tobacco budworm (Heilcoverpa armigera) larvae and all the larvae of H. armigera tested died during the first instar on the leaves of the mcbt-transformed lines whose expression level of CrylAb ICP exceeded 47.6 ng per 50 μg compared to the wild type CrylAb-transformed lines. Significantly higher feeding inhibition and/or growth inhibition of the insects was observed, compared to those on the non-transformed control plants.
- 日本育種学会の論文
著者
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Mochizuki Atsushi
Laboratory Of Applied Entomology Faculty Of Agriculture University Of Tokyo
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Mochizuki Atsushi
National Institute For Agro-environmental Sciences
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Komano Masayasu
Fukui Agricultural Experiment Station
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Shinoyama Harue
Fukui Agricultural Experiment Station
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Mochizuki Atsushi
ational Institute for Agro-Environmental Sciences
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Nomura Yukio
Fukui Agricultural Experiment Station
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Nagai Teruyuki
Fukui Agricultural Experiment Station
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Nomura Y
Fukui Agricultural Experiment Station
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Mochizuki A
National Institute For Agro-environmental Sciences
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Komano M
Fukui Agricultural Experiment Station
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