Ectopic Expression of an Oat Thionin Gene in Carnation Plants Confers Enhanced Resistance to Bacterial Wilt Disease
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概要
- 論文の詳細を見る
Under the control of Arabidopsis triptophan synthase β subunit promoter (PTSB1), the oat thionin gene (Asthi1) was introduced into carnation (Dianthus caryphyllus L.) via Agrobacterium-mediated gene transfer. Leaf bases of three cultivars of carnation (‘Scania’, ‘Percian Pink-Sim’ and ‘U. Conn. White Sim’) were infected with Agrobacterium harboring the binary vector with PTSB1::Asthi1 and hygromycin genes, and cultured on the medium containing hygromycin. Independent 99 lines resistant to hygromycin were regenerated and the introduction of the transgene was confirmed in 11 lines by polymerase chain reaction. Transgenic carnation plants exhibited the resistance against infection with Burkholderia caryophylli which caused bacterial wilt, and the levels of resistance correlated with those of the thionin gene transcript.
- 日本植物細胞分子生物学会の論文
- 2002-12-03
著者
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Nakamura Shigeo
Miyagi Prefectural Agriculture And Horticulture Research Center
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Seo S
Plant-microbe Interaction Research National Institute Of Agrobiological Sciences
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SHIRASAWA SEO
Miyagi Prefectural Agriculture and Horticulture Research Center
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UKAI Naomi
Miyagi Prefectural Agriculture and Horticulture Research Center
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HONKURA Ryoso
Miyagi Prefectural Agriculture and Horticulture Research Center
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IWAI Takayoshi
Miyagi Prefectural Agriculture and Horticulture Research Center
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OHASHI Yuko
Core Research for Evolutional Science and Technology
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IWAI Takayoshi
National Institute of Agrobiological Sciences
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SEO Naomi
Miyagi Prefectural Agricultural Research Center
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SHIRASAWA Naomi
Miyagi Prefectural Agricultural Research Center
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Shirasawa-seo Naomi
Miyagi Prefectural Agriculture And Horticulture Reseach Center
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Iwai Takayoshi
Miyagi Prefecture Agricultural Research Center
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Ohashi Yuko
Plant-microbe Interaction Research National Institute Of Agrobiological Sciences
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Seo Shigemi
Department Of Plant Physiology National Institute Of Agrobiological Sciences
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Ohashi Y
Department Of Molecular Genetics National Institute Of Agrobiological Resources
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Ohashi Yuko
Plant Physiol. Dept. National Institute Of Agrobiological Sciences
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Nakamura Shigeo
Miyagi Prefectural Agricultural Research Center
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OHASHI Yuko
Plant-Microbe Interactions Research Unit, National Institute of Agrobiological Sciences
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Honkura Ryoso
Miyagi Prefectural Agricultural Research Center
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