Immunochemical Localization of Pathogenesis-Related Proteins Secreted into the Intercellular Spaces of Salicylate-Treated Tobacco Leaves
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概要
- 論文の詳細を見る
In tobacco leaves, pathogenesis-related (PR) 1 proteins are abundantly induced by hypersensitive reaction to the infection with tobacco mosaic virus (TMV) and by treatment with salicylic acid, and are secreted into the intercellular spaces. To study the distribution of PR 1 proteins out-side of the cells, the immunogold technique was used with anti-PR 1 antibody. When sections of salicylate-treated tobacco leaf were reacted with antibody against PR 1a and then with protein A-gold complex, most of the gold label was localized in the intercellular spaces in the region between cells, and a little label was found in the cytoplasmic matrix and in small electron-dense granules in-side the cells. When salicylate-treated leaves were incubated with polygalacturonase and/or cellulase to liberate protoplasts or single cells from the tissue, most of PR 1 proteins were solubilized far before complete liberation of single cells, suggesting their localization in free spaces or a region susceptable to maceration, such as the secondary cell wall.
- 日本植物生理学会の論文
著者
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Ohashi Yuko
National Institute of Agrobiological Resources
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HOSOKAWA Daijiro
Faculty of Agriculture, Tokyo University of Agriculture and Technology
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Hosokawa Daijiro
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Ohashi Yuko
National Inst. Agrobiol. Resources:crest Of Japan Science And Technology Corporation
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