Localization of Pathogenesis-Related Proteins in the Epidermis and Intercellular Spaces of Tobacco Leaves after Their Induction by Potassium Salicylate or Tobacco Mosaic Virus Infection
スポンサーリンク
概要
- 論文の詳細を見る
The localization of pathogenesis-related (PR) proteins induced in tobacco leaves by treatment with potassium salicylate or a hypersensitive response to tobacco mosaic virus (TMV) infection was studied using immunochemical methods. Total PR protein levels increased with time after these treatments. The proportion of PR proteins in the intercellular spaces to the total content in the leaf discs rapidly rose in the later stage of the treatment to about 75% on the 9th day after salicylate treatment and to more than 80% on the 6th to 9th day after TMV inoculation. After 5 days of salicylate treatment, the amounts of PR proteins in the peeled leaf epidermis were twofold those in the mesophyll tissue. Only five percent or less of the total PR proteins in the epidermal and mesophyll tissues of salicylate-treated leaves were detected in the isolated epidermal and mesophyll protoplasts. The sugar content in highly purified PR 1a, 1b and 1c was less than one mole of monosaccharide per mole of each protein. These results show that the PR proteins are non-glycoproteins secreted into the intercellular spaces.
- 日本植物生理学会の論文
著者
-
Ohashi Yuko
National Institute of Agrobiological Resources
-
Matsuoka Makoto
National Institute of Agrobiological Resources
-
Ohashi Yuko
National Inst. Agrobiol. Resources:crest Of Japan Science And Technology Corporation
-
Matsuoka Makoto
National Agricultural Research Center For Kyushu-okinawa Region
-
Matsuoka Makoto
National Agricultural Res. Center For The Kyushu-okinawa Region National Agriculture And Food Res. O
関連論文
- Regulation by Cytokinins of Endogenous Levels of Jasmonic and Salicylic Acids in Mechanically Wounded Tobacco Plants
- Overexpression of a gene for 26S proteasome subunit RPN10 confers enhanced resistance to canavanine, an analog of arginine, in transgenic rice (Oryza sativa L.)
- A Calmodulin-Binding Mitogen-Activated Protein Kinase Phosphatase is Induced by Wounding and Regulates the Activities of Stress-Related Mitogen-Activated Protein Kinases in Rice
- Classification and Characterization of CDNA That Encodes the Light-Harvesting Chlorophyll a/b Binding Protein of Photosystem II from Rice
- Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
- Constitutive Promoters Available for Transgene Expression Instead of CaMV 35S RNA Promoter : Arabidopsis Promoters of Tryptophan Synthase Protein β Subunit and Phytochrome B
- Four Chitinase cDNAs from Chenopodium amaranticolor
- Efficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous Plants
- Structure and Characterization of a Gene for Light-Harvesting Chl a/b Binding Protein from Rice
- Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
- INVOLVEMENT OF PROTEASOME-UBIQUITIN SYSTEM IN WOUND-SIGNALING IN TOBACCO LEAVES
- Classification and Nucleotide Sequence of cDNA Encoding the Small Subunit of Ribulose-1,5-bisphosphate Carboxylase from Rice
- Enhanced Resistance to Salt, Cold and Wound Stresses by Overproduction of Animal Cell Death Suppressors Bcl-xL and Ced-9 in Tobacco Cells-Their Possible Contribution Through Improved Function of Organella
- EXPRESSION ANALYSIS OF TOBACCO CYSTEINE PROTEASE (NTCP-23)
- ISOLATION AND EXPRESSION ANALYSIS OF TOBACCO CYSTEINPROTEASE(NTCP-23)
- Immunological Studies on Pyruvate Orthophosphate Dikinase in C_3 Plants
- Localization of Pathogenesis-Related Proteins in the Epidermis and Intercellular Spaces of Tobacco Leaves after Their Induction by Potassium Salicylate or Tobacco Mosaic Virus Infection
- Regulation Mechanisms of the synthesis of Pathogenesis-Related Proteins in Tobacco Leaves
- The Presence of Two Types of Gene That Encode the Chlorophyll a/b-Binding Protein (LHCPII) and Their Light-Independent Expression in Pine (Pinus thunbergil)^1
- Induction and Secretion of Pathogenesis-Related Proteins by Salicylate or Plant Hormones in Tobacco suspension Cultures
- Induction of mRNAs for Phosphoenolpyruvate Carboxylase and Pyruvate, Orthophosphate Dikinase in Leaves of a C_3 Plant Exposed to Light
- Constitutive Expression of Stress-Inducible Genes, Including Pathogenesis-Related 1 Protein Gene in a Transgenic Interspecific Hybrid of Nicotiana glutinosa x Nicotiana debneyi
- Synthesis of Stress Proteins in Tabacco Leaves
- Ten Rice Peroxidases Redundantly Respond to Multiple Stresses Including Infection with Rice Blast Fungus
- Probenazole-Induced Accumulation of Salicylic Acid Confers Resistance to Magnaporthe grisea in Adult Rice Plants
- MAP Kinases Function Downstream of HSP90 and Upstream of Mitochondria in TMV Resistance Gene N-Mediated Hypersensitive Cell Death
- Accumulation of the Two Transcripts of the N gene, Conferring Resistance to Tobacco Mosaic Virus, is Probably Important for N Gene-dependent Hypersensitive Cell Death
- CHARACTERIZATION OF A TOBACCO WOUND-INDUCED RECEPTOR-TYPE PROTEIN KINASE GENE WRK1
- HYPERSENSITIVE CELL DEATH BY VIRUS INFECTION AND ACQUIRED RESISTANCE
- Enhanced Resistance to Bacterial Diseases of Transgenic Tobacco Plants Overexpressing Sarcotoxin IA, a Bactericidal Peptide of Insect
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- Enhanced Expression of an Antimicrobial Peptide Sarcotoxin IA by GUS Fusion in Transgenic Tobacco Plants
- Chemical composition and in situ degradability of two varieties of sugarcane at different growth stages in subtropical Japan
- Immunochemical Localization of Pathogenesis-Related Proteins Secreted into the Intercellular Spaces of Salicylate-Treated Tobacco Leaves
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- Transcriptional step is necessary for induction of pathogenesis-related proteins.
- Precise mode of induction of pathogenesis-related proteins by chemical treatment in tobacco.
- Enhanced Resistance to Bacterial Diseases of Transgenic Tobacco Plants Overexpressing Sarcotoxin IA, a Bactericidal Peptide of Insect.