Classification and Characterization of CDNA That Encodes the Light-Harvesting Chlorophyll a/b Binding Protein of Photosystem II from Rice
スポンサーリンク
概要
- 論文の詳細を見る
Thirteen cDNA clones for the light-harvesting chlorophyll a/b binding protein of photo-system II (LHCPII) from rice were constructed; they were grouped into two classes that were based on restriction enzyme maps. The nucleotide sequences from two clones that belonged to different classes were determined and compared. The clones contained a 798-bp and 792-bp open reading frame capable of coding for polypeptides with 265 and 263 amino acids, respectively. These sequences differ from each other, not only in the 5'-and 3'-noncoding regions, but also in the transit peptide regions and the N-terminal regions of the mature polypeptides. By contrast, the internal and C-terminal regions of the mature polypeptides are quite similar. It has been demonstrated that two types of LHCPII, with different structures and designated type I and type II, exist in some plants. A comparison of the deduced amino acid sequences between the rice clones and analogous sequences from other plants indicates that one of the pair of clones corresponds to type I LHCPII and the other to type II. Genomic Southern blot analysis using a specific probe for each type of LHCPII suggested that the genes for both types of protein are present as small multigene families in the rice genome. Northern blot analysis, using the specific probes, revealed that mRNAs for type I and type II LHCPII were barely detectable in dark-grown rice seedlings but they accumulated rapidly and in a similar manner after illumination of seedlings with white light. However, the amount of type I LHCPII mRNA was three times larger than that of type II mRNA in the greening seedlings. The results indicate that the mechanism for the regulation of the expression of the genes by light are similar but the amounts of mRNAs for type I and type II are different.
- 日本植物生理学会の論文
著者
-
Matsuoka M
Nagoya Univ. Nagoya Jpn
-
Matsuoka Makoto
National Institute of Agrobiological Resources
-
Matsuoka Makoto
National Agricultural Res. Center For The Kyushu-okinawa Region National Agriculture And Food Res. O
関連論文
- Classification and Characterization of CDNA That Encodes the Light-Harvesting Chlorophyll a/b Binding Protein of Photosystem II from Rice
- Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
- Structure and Characterization of a Gene for Light-Harvesting Chl a/b Binding Protein from Rice
- Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
- Classification and Nucleotide Sequence of cDNA Encoding the Small Subunit of Ribulose-1,5-bisphosphate Carboxylase from Rice
- Immunological Studies on Pyruvate Orthophosphate Dikinase in C_3 Plants
- Localization of Pathogenesis-Related Proteins in the Epidermis and Intercellular Spaces of Tobacco Leaves after Their Induction by Potassium Salicylate or Tobacco Mosaic Virus Infection
- Regulation Mechanisms of the synthesis of Pathogenesis-Related Proteins in Tobacco Leaves
- The Presence of Two Types of Gene That Encode the Chlorophyll a/b-Binding Protein (LHCPII) and Their Light-Independent Expression in Pine (Pinus thunbergil)^1
- Induction and Secretion of Pathogenesis-Related Proteins by Salicylate or Plant Hormones in Tobacco suspension Cultures
- Induction of mRNAs for Phosphoenolpyruvate Carboxylase and Pyruvate, Orthophosphate Dikinase in Leaves of a C_3 Plant Exposed to Light
- Constitutive Expression of Stress-Inducible Genes, Including Pathogenesis-Related 1 Protein Gene in a Transgenic Interspecific Hybrid of Nicotiana glutinosa x Nicotiana debneyi
- Synthesis of Stress Proteins in Tabacco Leaves
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- Chemical composition and in situ degradability of two varieties of sugarcane at different growth stages in subtropical Japan
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- Transcriptional step is necessary for induction of pathogenesis-related proteins.
- Precise mode of induction of pathogenesis-related proteins by chemical treatment in tobacco.