An Extracellular Insoluble Protein in Cultures and Seeds of Carrot : GROWTH AND DEVELOPMENT : PROTEINS, ENZYMES AND METABOLISM

概要

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An 18-kDa extracellular insoluble protein (EIP18) was found in the culture medium of calli and in seeds of carrot (Daucus carota L.). EIP18 was found in amorphous particles suspended in the culture medium of callus and was not solubilized by treatment of these particles with EDTA, with Triton X-100 plus NaCl or with LiCl, but it was partially solubilized by treatment with NaSCN and was entirely solubilized by treatment with urea. EIP18 seems not to belong to any known family of cell wall proteins because of the complete absence of glycan moieties and its low levels of hydroxyproline, proline and glycine. The protein in the medium seemed to have become insoluble as a result of formation of a homopolymer. In the plant, the protein was found only in the seed, being located both in the embryo and at the inner edge of the endosperm that faced the interspace between the embryo and the endosperm. EIP18 might be an extracellular matrix protein specific to seeds.
日本植物生理学会の論文