Studies of Diabetic Nephropathy. I. Effects of Storage Time and Temperature on Microalbuminuria

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The effect of storage time and temperature on the immunological turbidimetric measurement of a low concentration of albumin in urine was investigated. In storage at -20℃, the albumin level decreased, but the rate of this decrease differed considerably among specimens. However, under storage at room temperature for 2 weeks, or at 4℃ for 5 weeks albumin levels did not show significant changes. At -40℃ and -80℃ there were only slight decreases. At -40℃ decreases were slightly greater than at -80℃. Therefore, -80℃ was found to be the optimal temperature for long-term storage of urinary albumin. Some of the specimens showed a 50% decrease in albumin level after storage for 9 weeks at -20℃, but remained unchanged after storage for the same period at -80℃. A pair of specimens preserved at -20℃ and -80℃ were isolated by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). After electrophoresis, urinary proteins were stained by silver staining to observe bands, and albumin content was determined by immunoblotting. A decrease in albumin concentration was also observed by densitometric detection.
社団法人日本薬学会の論文
1994-09-15