Influence of Sugar Chain on Fibrin Affinity of Recombinat t-PA

概要

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The role of the sugar chain on the fibrin affinity property of tissue plasminogen activator(t-PA)was investigated using two variants of wild type t-PA(WT t-PA I and WT t-PA II)and mutant type t-PA(mt-PA ; Gln117 t-PA I and Gln117 t-PA II), whose sugar chains have different structures. In terms of fibrin affinity, Gln117 t-PA was higher than WT t-PA ; moreover, Type II was higher than Type I. Bindings mediated via finger domain(F mode)and kringle 2 domain(K2 mode)were distinguished using ε-amino caproic acid(EACA). Consequently, F mode and K2 mode bindings were inhibited by the sugar chains at Asn117 and 184, respectively. These results were assumed to be due to the steric hindrance of the sugar chains.
公益社団法人日本薬学会の論文
2001-03-01